Rocco Maria Luisa, Balzamino Bijorn Omar, Petrocchi Passeri Pamela, Micera Alessandra, Aloe Luigi
Institute of Cell Biology and Neurobiology, CNR, Rome, Italy.
IRCCS-G.B. Bietti Foundation, Rome, Italy.
PLoS One. 2015 Apr 21;10(4):e0124810. doi: 10.1371/journal.pone.0124810. eCollection 2015.
A number of different studies have shown that neurotrophins, including nerve growth factor (NGF) support the survival of retinal ganglion neurons during a variety if insults. Recently, we have reported that that eye NGF administration can protect also photoreceptor degeneration in a mice and rat with inherited retinitis pigmentosa. However, the evidence that NGF acts directly on photoreceptors and that other retinal cells mediate the NGF effect could not be excluded. In the present study we have isolated retinal cells from rats with inherited retinitis pigmentosa (RP) during the post-natal stage of photoreceptor degenerative. In presence of NGF, these cells are characterized by enhanced expression of NGF-receptors and rhodopsin, the specific marker of photoreceptor and better cell survival, as well as neuritis outgrowth. Together these observations support the hypothesis that NGF that NGF acts directly on photoreceptors survival and prevents photoreceptor degeneration as previously suggested by in vivo studies.
多项不同研究表明，包括神经生长因子（NGF）在内的神经营养因子在多种损伤情况下可支持视网膜神经节神经元的存活。最近，我们报道了向眼部施用NGF也可保护患有遗传性视网膜色素变性的小鼠和大鼠的光感受器免于退化。然而，不能排除NGF直接作用于光感受器以及其他视网膜细胞介导NGF效应的证据。在本研究中，我们在光感受器退化的产后阶段从患有遗传性视网膜色素变性（RP）的大鼠中分离出视网膜细胞。在有NGF存在的情况下，这些细胞的特征是NGF受体和视紫红质（光感受器的特异性标志物）的表达增强、细胞存活情况更好以及神经突生长。这些观察结果共同支持了以下假设，即如先前体内研究所表明的，NGF直接作用于光感受器的存活并防止光感受器退化。
