Dean Kevin M, Davis Lloyd M, Lubbeck Jennifer L, Manna Premashis, Friis Pia, Palmer Amy E, Jimenez Ralph
†BioFrontiers Institute, University of Colorado, Boulder, Colorado 80309, United States.
‡Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309, United States.
Anal Chem. 2015;87(10):5026-30. doi: 10.1021/acs.analchem.5b00607. Epub 2015 Apr 29.
There is a critical need for high-speed multiparameter photophysical measurements of large libraries of fluorescent probe variants for imaging and biosensor development. We present a microfluidic flow cytometer that rapidly assays 10(4)-10(5) member cell-based fluorophore libraries, simultaneously measuring fluorescence lifetime and photobleaching. Together, these photophysical characteristics determine imaging performance. We demonstrate the ability to resolve the diverse photophysical characteristics of different library types and the ability to identify rare populations.
对于用于成像和生物传感器开发的大量荧光探针变体文库，迫切需要进行高速多参数光物理测量。我们展示了一种微流控流式细胞仪，它可以快速检测基于细胞的10⁴-10⁵个荧光团文库，同时测量荧光寿命和光漂白。这些光物理特性共同决定了成像性能。我们展示了分辨不同文库类型多样光物理特性的能力以及识别稀有群体的能力。
