啮齿维永氏菌细胞上噬菌体受体的纯化与特性分析。维永氏菌上的噬菌体受体。

Purification and characterization of bacteriophage receptor on Veillonella rodentium cells. Phage-receptor on Veillonella.

作者信息

Totsuka M, Ono T

机构信息

Department of Oral Bacteriology, Nagasaki University School of Dentistry, Japan.

出版信息

Antonie Van Leeuwenhoek. 1989 Oct;56(3):263-71. doi: 10.1007/BF00418938.

DOI:10.1007/BF00418938

PMID:2589854

Abstract

Veillonellophage N2 adsorbed to polysaccharides (PSs) on Veillonella rodentium ATCC 17743 cell wall, and the bacteriophage receptor contained only glucosamine. D(+)-glucosamine hydrochloride (Sigma) also adsorbed the veillonellophage N2. These results therefore indicate that the receptor to the veillonellophage N2 is cell wall PSs. The PSs of the host cells as receptor have been characterized. Glucosamine accounted for approximately 100% of the weight of the PSs. The PSs which were partially resolved by Sephadex G-75 chromatography comprised approximately four glucosamine units. Their primary structure was determined by 400 MHz n.m.r. spectroscopy. One- and two-dimensional 1H-nmr experiments showed the PS to be a branched polymer. Glucosamine linkage was detected in one of the branches.

摘要

啮蚀艾肯菌噬菌体N2吸附于啮蚀艾肯菌ATCC 17743细胞壁上的多糖（PSs），且该噬菌体受体仅含氨基葡萄糖。盐酸D(+)-氨基葡萄糖（西格玛）也能吸附啮蚀艾肯菌噬菌体N2。因此，这些结果表明啮蚀艾肯菌噬菌体N2的受体是细胞壁PSs。已对作为受体的宿主细胞PSs进行了表征。氨基葡萄糖约占PSs重量的100%。经葡聚糖G-75柱色谱法部分分离的PSs约由四个氨基葡萄糖单元组成。其一级结构通过400 MHz核磁共振光谱法测定。一维和二维1H-核磁共振实验表明该PS为支链聚合物。在其中一个分支中检测到了氨基葡萄糖连接。