[屋尘螨变应原Der f1和Der f2在本氏烟草叶片中的表达]

[[Expression of house dust mite allergens Der f1 and Der f2 in Nicotiana benthamiana leaves].

作者信息

Riazantsev D Iu, Drobiazina P E, Hlgatian S V, Zavriev S K, Svirshchevskaia E V

出版信息

Bioorg Khim. 2014 Jul-Aug;40(4):468-78.

Abstract

The class E immunoglobulins (IgE) is known to recognize conformational epitopes and therefore the native conformation of recombinant allergens is essential for their using in test-systems. Recombinant Dermatophagoides farinae house dust mite (HDM) allergens Der f1 and Der f2 were expressed in bacteria Escherichia coli and Nicotiana benthamiana plants. It has been shown that IgE in sera from children allergic to HDM recognizes Der f2 expressed both in E. coli and N. benthamiana. Mature form of Der f1 expressed in E. coli does not interact with IgE while the protein purified from N. benthamiana is able to recognize IgE as a native allergen.

摘要

已知E类免疫球蛋白（IgE）可识别构象表位，因此重组变应原的天然构象对于其在检测系统中的应用至关重要。重组粉尘螨变应原Der f1和Der f2在大肠杆菌和本氏烟草植物中表达。研究表明，对粉尘螨过敏儿童血清中的IgE可识别在大肠杆菌和本氏烟草中表达的Der f2。在大肠杆菌中表达的成熟形式的Der f1不与IgE相互作用，而从本氏烟草中纯化的蛋白能够作为天然变应原识别IgE。