Coetzee K, Kruger T F, Menkveld R, Lombard C J, Swanson R J
Infertility Clinic, Tygerberg Hospital, South Africa.
Arch Androl. 1989;23(2):131-8. doi: 10.3109/01485018908986835.
The hypoosmotic swelling test (HOS) is a simple test to measure the functional competence of human sperm membranes. The question is, does a relationship exist between this competence and the fertilization potential of human spermatozoa? In this study the strongest correlation (r = 0.76) was obtained between sperm swelling and sperm viability (supravital staining). Only a moderate correlation (r = 0.50) was obtained with normal sperm morphology; weaker correlations were also obtained with the sperm penetration assay (r = 0.42) and human IVF (r = 0.24). The results, therefore, indicate that the HOS test has a limited predictive value. Notwithstanding this low concordance between sperm swelling and fertilizing potential, a less than 50% HOS test threshold was seen to be a definite indicator of a male factor.
低渗肿胀试验（HOS）是一种用于测量人类精子膜功能能力的简单试验。问题在于，这种能力与人类精子的受精潜力之间是否存在关联？在本研究中，精子肿胀与精子活力（体外活体染色）之间获得了最强的相关性（r = 0.76）。与正常精子形态仅获得了中等程度的相关性（r = 0.50）；与精子穿透试验（r = 0.42）和人类体外受精（r = 0.24）也获得了较弱的相关性。因此，结果表明HOS试验的预测价值有限。尽管精子肿胀与受精潜力之间的一致性较低，但HOS试验阈值低于50%被视为男性因素的明确指标。
