Chan S Y, Fox E J, Chan M M, Tsoi W L, Wang C, Tang L C, Tang G W, Ho P C
Fertil Steril. 1985 Nov;44(5):668-72. doi: 10.1016/s0015-0282(16)48985-5.
The functional integrity of sperm membranes of 270 semen samples collected from fertile men and the male partners in couples with infertile marriages was assessed by the hypoosmotic swelling test and the results correlated with routine semen analysis and the human sperm zona-free hamster ovum penetration assay. Semen samples with abnormal semen parameters had lower values of percentage of swollen sperm after hypoosmotic treatment in comparison with those with normal semen parameters. A weak positive correlation was observed between sperm swelling and sperm morphologic features (r = 0.32, P less than 0.05) and between sperm swelling and sperm motility (r = 0.22, P less than 0.05). Insignificant correlation was observed between sperm swelling and in vitro sperm fertilizing capacity, as assessed by the zona-free hamster ovum penetration assay. The results indicate that the sperm swelling test and the zona-free hamster ovum penetration assay are evaluating different functional qualities of sperm that are apparently not associated with each other.
通过低渗肿胀试验评估了从生育能力正常的男性以及不育夫妇中的男性伴侣采集的270份精液样本的精子膜功能完整性，并将结果与常规精液分析及人精子无透明带仓鼠卵穿透试验进行了关联。与精液参数正常的样本相比，精液参数异常的样本在低渗处理后肿胀精子的百分比值更低。在精子肿胀与精子形态特征之间观察到弱正相关（r = 0.32，P < 0.05），在精子肿胀与精子活力之间也观察到弱正相关（r = 0.22，P < 0.05）。通过无透明带仓鼠卵穿透试验评估发现，精子肿胀与体外精子受精能力之间无显著相关性。结果表明，精子肿胀试验和无透明带仓鼠卵穿透试验评估的是精子不同的功能特性，这些特性显然彼此不相关。
