Ghafoor Tahir, Ikram Aamer, Abbasi Shahid Ahmad, Zaman Gohar, Ayyub Muhammad, Palomino Juan Carlos, Vandamme Peter, Martin Anandi
Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
Department of Haematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi.
J Coll Physicians Surg Pak. 2015 Apr;25(4):250-3.
To determine the current sensitivity pattern of second line anti-tuberculosis drugs against clinical isolates of Multidrug Resistant Mycobacterium tuberculosis (MDR-TB).
A cross-sectional study.
Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from November 2011 to April 2013.
Samples received during the study period were processed on BACTEC MGIT 960 system for Mycobacterium tuberculosis (MTB) culture followed by first line drugs susceptibility testing of culture proven MTB isolates. On the basis of resistance to rifampicin and isoniazid, 100 clinical isolates of MDR-TB were further subjected to susceptibility testing against amikacin (AMK), capreomycin (CAP), ofloxacin (OFL) and ethionamide (ETH) as per standard BACTEC MGIT 960 instructions.
Out of 100 MDR-TB isolates, 62% were from male patients and 38% from female patients. 97% were sensitive to AMK, 53% to OFL, 87% to CAP; and 87% were sensitive to ETH.
The majority of the MDR-TB isolates showed excellent sensitivity against AMK, CAP and ETH. However, sensitivity of MDR-TB isolates against fluoroquinolones like OFL was not encouraging.
确定二线抗结核药物对耐多药结核分枝杆菌（MDR-TB）临床分离株的当前敏感性模式。
横断面研究。
2011年11月至2013年4月，拉瓦尔品第武装部队病理研究所（AFIP）微生物学系。
在研究期间收到的样本在BACTEC MGIT 960系统上进行结核分枝杆菌（MTB）培养，随后对培养证实的MTB分离株进行一线药物敏感性测试。根据对利福平和平的耐药性，按照标准的BACTEC MGIT 960说明，对100株MDR-TB临床分离株进一步进行对阿米卡星（AMK）、卷曲霉素（CAP）、氧氟沙星（OFL）和乙硫异烟胺（ETH）的敏感性测试。
在100株MDR-TB分离株中，62%来自男性患者，38%来自女性患者。97%对AMK敏感，53%对OFL敏感，87%对CAP敏感；87%对ETH敏感
大多数MDR-TB分离株对AMK、CAP和ETH表现出优异的敏感性。然而，MDR-TB分离株对OFL等氟喹诺酮类药物的敏感性并不乐观。
