Bae Yong Chan, Song Ji Sun, Bae Seong Hwan, Kim Joo Hyoung
*Department of Plastic and Reconstructive Surgery, Pusan National University School of Medicine, Busan, Korea; †Biomedical Research Institute, Pusan National University Hospital, Busan, Korea.
Dermatol Surg. 2015 May;41(5):605-14. doi: 10.1097/DSS.0000000000000342.
The use of cryopreserved adipose tissue for soft tissue augmentation is common, but unpredictability of fat graft viability remains a limitation. Adipose-derived stem cell (ADSC) and stromal vascular fraction (SVF) have been introduced to enhance viability and improve the survival of transplanted fat tissue.
To investigate whether supplementation with ADSC or SVF improved the survival of cryopreserved fat grafts.
The cryopreserved fat grafts were treated with ADSC, SVF, or normal saline in 30 six-week-old male nude mice to test whether ADSC and SVF could improve the survival of the transplanted fat tissue. The authors examined the weight, volume, and histological features of each group (n = 10) at 8 weeks after transplantation to evaluate the survival of the fat tissue.
There was no difference between the control and SVF groups with respect to weight, volume, and histological findings. However, the ADSC group showed a significant increase in weight and volume compared with the control and SVF groups. Histological examination showed that the ADSC supplementation improved the quality of the transplanted fat grafts.
Taken together, these results suggest a potential clinical utility of ADSC but no advantage of SVF in facilitating cryopreserved fat transfer.
使用冷冻保存的脂肪组织进行软组织填充很常见，但脂肪移植存活率的不可预测性仍然是一个限制因素。脂肪来源干细胞（ADSC）和基质血管成分（SVF）已被引入以提高移植脂肪组织的存活率并改善其存活情况。
研究补充ADSC或SVF是否能提高冷冻保存脂肪移植的存活率。
将冷冻保存的脂肪移植体用ADSC、SVF或生理盐水处理，植入30只六周龄雄性裸鼠体内，以测试ADSC和SVF是否能提高移植脂肪组织的存活率。作者在移植后8周检查每组（n = 10）的重量、体积和组织学特征，以评估脂肪组织的存活情况。
对照组和SVF组在重量、体积和组织学结果方面没有差异。然而，与对照组和SVF组相比，ADSC组的重量和体积显著增加。组织学检查表明，补充ADSC改善了移植脂肪移植物的质量。
综上所述，这些结果表明ADSC具有潜在的临床应用价值，但在促进冷冻保存脂肪移植方面，SVF并无优势。
