Gao Li, Peng Xiao-Ming, Huo Shi-Xia, Liu Xin-Ming, Yan Ming
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uighur Medicine, Urumqi, 830049, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, 100193, China.
Phytother Res. 2015 Aug;29(8):1131-6. doi: 10.1002/ptr.5357. Epub 2015 Apr 21.
Acteoside, also known as verbascoside or orobanchin, is a common compound found in many important medicinal plants including the Chinese herb Cistanche deserticola Y. C. Ma, which is used for its neuroprotective and memory enhancement properties. We have investigated the effects of acteoside using a senescent mouse model induced by a combination of chronic intraperitoneal administration of d-gal (60 mg/kg/day) and oral administration AlCl3 (5 mg/kg/day) once daily for 90 days. After 60 days, acteoside (30, 60, and 120 mg/kg/day) was orally administered once daily for 30 days. The memory enhancing effects of acteoside were evaluated using the Morris water maze test. The results showed that 30-120 mg/kg/day of acteoside reduced the escape latency in finding the platform, and increased the number of crossings of the platform. A 30-120 mg/kg/day of acteoside increased significantly the expression of nerve growth factor and tropomycin receptor kinase A mRNA and protein in the hippocampus, measured using real-time RT-PCR, immunohistochemical analysis, and western blotting. These results support the use of C. deserticola for memory enhancement and indicate that the effects of acteoside are induced via promotion of nerve growth factor and tropomycin receptor kinase A expression.
毛蕊花糖苷，也被称为麦角硫因或肉苁蓉苷，是一种常见的化合物，存在于许多重要的药用植物中，包括中国草药肉苁蓉，肉苁蓉因其神经保护和增强记忆的特性而被使用。我们使用了一种衰老小鼠模型来研究毛蕊花糖苷的作用，该模型通过连续90天每天腹腔注射d-半乳糖（60毫克/千克/天）和口服氯化铝（5毫克/千克/天）诱导而成。60天后，每天口服毛蕊花糖苷（30、60和120毫克/千克/天），持续30天。使用莫里斯水迷宫试验评估毛蕊花糖苷的记忆增强作用。结果表明，每天30 - 120毫克/千克的毛蕊花糖苷可缩短找到平台的逃避潜伏期，并增加穿越平台的次数。使用实时逆转录聚合酶链反应、免疫组织化学分析和蛋白质印迹法测量，每天30 - 120毫克/千克的毛蕊花糖苷可显著增加海马体中神经生长因子和原肌球蛋白受体激酶A mRNA及蛋白质的表达。这些结果支持将肉苁蓉用于增强记忆，并表明毛蕊花糖苷的作用是通过促进神经生长因子和原肌球蛋白受体激酶A的表达而诱导产生的。
