Gao Li, Peng Xiaoming, Huo Shixia, He Yan, Yan Ming
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Prescription Laboratory of Xinjiang Traditional Uyghur Medicine, Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Oct;30(10):1022-5.
To study the effect of acteoside on the expression of neurotrophin-3 (NT-3) in brain tissues of subacute aging mice induced by D-galactose (D-Gal) combined with aluminum trichloride (AlCl3).
Female Kunming mice were randomly divided into vehicle control group, D-Gal combined with AlCl3 group , vitamin E group, piracetam group and acteoside groups [30, 60, 120 mg/(kg.d)]. Mice in D-Gal combined with AlCl3, vitamin E, piracetam and acteoside groups were injected intraperitoneally (i.p.) with D-Gal [60 mg/(kg.d)] and AlCl3 [5 mg/(kg.d)] for 90 days to induce the subacute aging models. The real-time quantitative PCR, Western blotting and immunohistochemistry were respectively used to detect the expression of NT-3 mRNA and protein in cerebral cortex and hippocampus of mouse brain.
D-Gal and AlCl3 caused a significant reduction of NT-3 in cerebral cortex and hippocampus. Acteoside, vitamin E and piracetam increased the decreased expression of NT-3 induced by D-Gal and AlCl3, and the difference was statistically significant (P<0.05 or P<0.01).
Acteoside can up-regulate the expression of brain NT-3 in D-Gal plus AlCl3 treated mice.
研究毛蕊花糖苷对D-半乳糖（D-Gal）联合三氯化铝（AlCl3）诱导的亚急性衰老小鼠脑组织中神经营养因子-3（NT-3）表达的影响。
将雌性昆明小鼠随机分为溶剂对照组、D-Gal联合AlCl3组、维生素E组、吡拉西坦组和毛蕊花糖苷组[30、60、120mg/（kg·d）]。D-Gal联合AlCl3组、维生素E组、吡拉西坦组和毛蕊花糖苷组小鼠腹腔注射D-Gal[60mg/（kg·d）]和AlCl3[5mg/（kg·d）]，连续90天以诱导亚急性衰老模型。分别采用实时定量PCR、蛋白质印迹法和免疫组织化学法检测小鼠脑皮质和海马中NT-3mRNA和蛋白的表达。
D-Gal和AlCl3导致脑皮质和海马中NT-3显著降低。毛蕊花糖苷、维生素E和吡拉西坦增加了D-Gal和AlCl3诱导的NT-3降低的表达，差异具有统计学意义（P<0.05或P<0.01）。
毛蕊花糖苷可上调D-Gal加AlCl3处理小鼠脑内NT-3的表达。
