Zhu Wenjie, Fan Sigang, Huang Guiju, Zhang Dongling, Liu Baosuo, Bi Xiaomin, Yu Dahui
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Division of Aquaculture and Biotechnology, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Gene. 2015 Jul 25;566(2):201-11. doi: 10.1016/j.gene.2015.04.046. Epub 2015 Apr 18.
Epidermal growth factor receptor (EGFR) plays an important role in cell growth, proliferation, differentiation and migration. Yet whether it functions in pearl formation or not is not reported. In this study, EGFR was cloned from the pearl oyster Pinctada fucata (named as Pf-EGFR) and its expression profiles were investigated. The cDNA was 2156bp long with an ORF of 1017bp encoding 338 amino acid residues. The deduced polypeptide contained an L domain and a cysteine-rich domain, consistent with the characteristics of ErbB family. The sequence of Pf-EGFR shared 22.78-56.71% identity with other EGFRs. The genomic sequence of Pf-EGFR consisted of six exons and five introns, being 5190bp in total length, and expressed in all the tested tissues with a higher expression level in the pearl sac (P<0.05). In situ hybridization showed that Pf-EGFR was specifically expressed on both the inner side of the outer fold and the outer side of the inner fold of the mantle, as well as on the whole pearl sac including the connective tissues. In addition, Pf-EGFR was markedly increased at larval metamorphosis, significantly higher than other development periods (P<0.05). These results indicated that the Pf-EGFR may facilitate pearl formation as well as larval metamorphosis.
表皮生长因子受体（EGFR）在细胞生长、增殖、分化和迁移中发挥着重要作用。然而，其是否在珍珠形成过程中发挥作用尚未见报道。在本研究中，从合浦珠母贝中克隆了EGFR（命名为Pf-EGFR）并对其表达谱进行了研究。该cDNA长2156bp，开放阅读框为1017bp，编码338个氨基酸残基。推导的多肽包含一个L结构域和一个富含半胱氨酸的结构域，与ErbB家族的特征一致。Pf-EGFR的序列与其他EGFR的序列一致性为22.78-56.71%。Pf-EGFR的基因组序列由6个外显子和5个内含子组成，全长5190bp，在所有检测组织中均有表达，在珍珠囊中表达水平较高（P<0.05）。原位杂交显示，Pf-EGFR在外套膜外褶内侧和内褶外侧以及包括结缔组织在内的整个珍珠囊中特异性表达。此外，Pf-EGFR在幼虫变态时显著增加，明显高于其他发育时期（P<0.05）。这些结果表明，Pf-EGFR可能促进珍珠形成以及幼虫变态。
