Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
J Environ Manage. 2015 Jun 1;156:290-8. doi: 10.1016/j.jenvman.2015.03.045. Epub 2015 Apr 19.
The kitchen waste fraction in municipal solid waste contains high organic matter particularly carbohydrate that can contribute to fermentable sugar production for subsequent conversion to bioethanol. This study was carried out to evaluate the influence of single and combination pretreatments of kitchen waste by liquid hot water, mild acid pretreatment of hydrochloric acid (HCl) and sulphuric acid (H2SO4) and enzymatic hydrolysis (glucoamylase). The maximum total fermentable sugar produced after combination pretreatment by 1.5% HCl and glucoamylase consisted of 93.25 g/L glucose, 0.542 g/L sucrose, 0.348 g/L maltose, and 0.321 g/L fructose. The glucose released by the combination pretreatment method was 0.79 g glucose/g KW equivalent to 79% of glucose conversion. The effects of the pre-treatment on kitchen waste indicated that the highest solubilization was 40% by the combination method of 1.5% HCl and glucoamylase. The best combination pre-treatment gave concentrations of lactic acid, acetic acid, and propionic acid of 11.74 g/L, 6.77 g/L, and 1.02 g/L, respectively. The decrease of aliphatic absorbance bands of polysaccharides at 2851 and 2923 cm(-1) and the increase on structures of carbonyl absorbance bands at 1600 cm(-1) reflects the progress of the kitchen waste hydrolysis to fermentable sugars. Overall, 1.5% HCl and glucoamylase treatment was the most profitable process as the minimum selling price of glucose was USD 0.101/g kitchen waste. Therefore, the combination pretreatment method was proposed to enhance the production of fermentable sugar, particularly glucose from kitchen waste as the feedstock for bioethanol production.
厨余垃圾在城市固体废弃物中含有大量有机物质，特别是碳水化合物，可以作为发酵糖生产的原料，进而转化为生物乙醇。本研究旨在评估单独和联合预处理厨余垃圾的影响，预处理方法包括热水、盐酸（HCl）和硫酸（H2SO4）的温和酸预处理以及酶水解（糖化酶）。经 1.5% HCl 和糖化酶联合预处理后，最大的总可发酵糖产量为 93.25 g/L 葡萄糖、0.542 g/L 蔗糖、0.348 g/L 麦芽糖和 0.321 g/L 果糖。组合预处理方法释放的葡萄糖为 0.79 g 葡萄糖/gKW，相当于 79%的葡萄糖转化率。预处理对厨余垃圾的影响表明，HCl 和糖化酶联合法的最高溶解率为 40%。最佳的联合预处理方法分别产生 11.74 g/L、6.77 g/L 和 1.02 g/L 的乳酸、乙酸和丙酸。2851 和 2923 cm(-1) 处多糖的脂肪族吸收带的减少和 1600 cm(-1) 处羰基吸收带的增加反映了厨余垃圾水解为可发酵糖的进展。总体而言，1.5% HCl 和糖化酶处理是最有利可图的工艺，因为葡萄糖的最低销售价格为 0.101 美元/g 厨余垃圾。因此，提出了联合预处理方法，以提高可发酵糖（特别是葡萄糖）的产量，将厨余垃圾作为生物乙醇生产的原料。
