在AS-7中储存洗涤过的或经辐照的红细胞可改善其体外特性。

Storage of washed or irradiated red cells in AS-7 improves their in vitro characteristics.

作者信息

Proffitt S, Thomas S, Swann I, Popovsky M A, Smith D J, Roberts D J, Cardigan R

机构信息

Component Development Laboratory, NHS Blood and Transplant, Cambridge, UK.

Haemonetics Corporation, Braintree, MA, USA.

出版信息

Vox Sang. 2015 Oct;109(3):203-13. doi: 10.1111/vox.12271. Epub 2015 Apr 20.

DOI:10.1111/vox.12271

PMID:25900147

Abstract

BACKGROUND

AS-7 is a new alkaline hypotonic red cell additive solution (AS) shown to improve red cell quality during storage compared with AS-1. We sought to compare red cells stored in AS-7 with those stored in SAGM using RCC that were either untreated, or washed or irradiated on day 14 of storage.

STUDY DESIGN AND METHODS

A pooled and split study design was used to produce seven identical RCC (four in SAGM and three in AS-7). At day 14 following donation, two RCC (one in SAGM and one in AS-7) were gamma irradiated and three RCC (two in SAGM and one in AS-7) were washed and resuspended in either SAGM or AS-7. RCC were sampled for analysis throughout storage and at end of shelf life: day 28 for washed or irradiated and day 35 for untreated RCC.

RESULTS

For untreated, washed or irradiated RCC, those stored in AS-7 had lower haemolysis, red cell microvesicles and supernatant potassium content than RCC in SAGM. In addition, ATP levels and pH were better maintained in AS-7 RCC than in SAGM RCC.

CONCLUSION

These data suggest that the quality of these components may be improved by storage in AS-7 compared with SAGM.

摘要

背景

AS-7是一种新型碱性低渗红细胞添加液，与AS-1相比，已证明其在储存期间可改善红细胞质量。我们试图比较储存在AS-7中的红细胞与储存在SAGM中的红细胞，这些红细胞来自储存第14天未处理、洗涤或辐照的红细胞悬液（RCC）。

研究设计与方法

采用汇集和分割研究设计来制备7个相同的RCC（4个在SAGM中，3个在AS-7中）。在献血后第14天，对2个RCC（1个在SAGM中，1个在AS-7中）进行γ辐照，对3个RCC（2个在SAGM中，1个在AS-7中）进行洗涤并重悬于SAGM或AS-7中。在整个储存期间和保质期结束时对RCC进行采样分析：洗涤或辐照的RCC在第28天，未处理的RCC在第35天。