Alzolibani Abdullateef A, Settin Ahmed, Ahmed Ahmed Ali, Ismail Hisham, Elhefni Noor, Al Robaee Ahmad A
Department of Dermatology, College of Medicine, Qassim University, Saudi Arabia.
Research Centre, College of Medicine, Qassim University, Saudi Arabia.
Int J Health Sci (Qassim). 2015 Jan;9(1):25-33. doi: 10.12816/0024680.
Nuclear factor-κB (NF-κB) and small ubiquitin-like modifier (SUMO4) are key transcription factors involved in the regulation of immune responses and apoptosis. The aim of this study is to test for the association of NF-κB and SUMO gene polymorphisms with the susceptibility and severity of psoriasis among Saudi cases.
This is a case controlled study including 85 Saudi psoriasis patients in addition to 92 matched healthy unrelated controls from the same locality. For all participants, DNA was analyzed by PCR for characterization of NF-κB1 -94 del/ins ATTG, NF-κB IA 2758 A>G and SUMO rs237025 G>A gene polymorphisms.
Compared to controls, psoriasis patients showed a non-significant difference for all frequencies of genotypes and alleles of NF-κB1 ins/del, NF-κB1A A>G and SUMO4 G>A polymorphisms (p>0.05). However, cases with the plaque type had significantly higher frequency of the SUMO4 A allele carriage (GA+AA genoytpes) than the guttate type (78.6% vs. 21.4%, p=0.02). The PASI score was also significantly higher among cases with the NF-κB1A AA genotype than other cases (p=0.00).
Genetic polymorphisms of NF-κB1-94 ins/del ATTG, NF-κB IA 2758 A>G and SUMO4 rs237025 G>A were not associated with the susceptibility to psoriasis vulgaris in Saudi patients. However, it might be associated with the expressivity of the disease in terms of its clinical type and severity.
核因子-κB（NF-κB）和小泛素样修饰物（SUMO4）是参与免疫反应和细胞凋亡调节的关键转录因子。本研究旨在检测沙特患者中NF-κB和SUMO基因多态性与银屑病易感性及严重程度之间的关联。
这是一项病例对照研究，除了92名来自同一地区的匹配健康无关对照者外，还纳入了85名沙特银屑病患者。对所有参与者的DNA进行聚合酶链反应（PCR）分析，以鉴定NF-κB1 -94 del/ins ATTG、NF-κB IA 2758 A>G和SUMO rs237025 G>A基因多态性。
与对照组相比，银屑病患者在NF-κB1 ins/del、NF-κB1A A>G和SUMO4 G>A多态性的所有基因型和等位基因频率上均无显著差异（p>0.05）。然而，斑块型患者中SUMO4 A等位基因携带（GA + AA基因型）的频率显著高于点滴型患者（78.6% 对21.4%，p = 0.02）。NF-κB1A AA基因型的患者的银屑病面积和严重程度指数（PASI）评分也显著高于其他患者（p = 0.00）。
NF-κB1-94 ins/del ATTG、NF-κB IA 2758 A>G和SUMO4 rs237025 G>A基因多态性与沙特患者寻常型银屑病的易感性无关。然而，就疾病的临床类型和严重程度而言，它可能与疾病的表现相关。
