Swain Susree A, Sarangi Pratikhya, Rattan Roma, Sahu Pratima K, Lamare Andrew A
Biochemistry, SCB Medical College, Cuttack, IND.
Biochemistry, SCB Medical College and Hospital, Cuttack, IND.
Cureus. 2022 Apr 22;14(4):e24401. doi: 10.7759/cureus.24401. eCollection 2022 Apr.
Background and objective Schizophrenia is a chronic mental illness that is associated with multifactorial causation, but the greatest risk factor is a positive family history. Previous studies have suggested that proinflammatory cytokines and acute-phase proteins increase and modulate the severity of symptoms of schizophrenia. The inflammatory milieu in these patients has been found to be controlled by the transcription factor nuclear factor-kappa B1 (NF-κB1) in the inflammatory cells. In this study, we aimed to examine the correlation between polymorphism of the NF-κB1 gene and the severity of disease symptoms in schizophrenia patients. Materials and methods This was a case-control study conducted on 90 diagnosed cases of schizophrenia patients with 90 matched healthy volunteers as controls. DNA was extracted from EDTA blood samples and PCR was run, and the study of the NF-κB1 gene polymorphism rs28362691 (-94 ATTG ins/del) was performed by using restriction fragment length polymorphism (RFLP). Results We observed the ins/ins genotype (78%) to be more prevalent among the study population. The del/del and ins/del genotypes were seen in 6.7% and 14.4% of schizophrenic patients respectively. The insertion allele was seen more than the deletion allele. Pearson's correlation analysis showed a significant positive correlation between NF-κB1 levels and disease severity with an r-value of 0.471 and a p-value of 0.027. Conclusion We found that in schizophrenia patients, the insertion allele was higher than the deletion allele and the ins/ins genotype was higher in frequency than the del/del and ins/del genotypes. There was a strong positive association between the insertion genotype and the severity of disease symptoms in schizophrenia patients.
精神分裂症是一种慢性精神疾病，其病因是多因素的，但最大的风险因素是家族遗传史阳性。先前的研究表明，促炎细胞因子和急性期蛋白会增加并调节精神分裂症症状的严重程度。已发现这些患者的炎症环境由炎症细胞中的转录因子核因子-κB1（NF-κB1）控制。在本研究中，我们旨在研究NF-κB1基因多态性与精神分裂症患者疾病症状严重程度之间的相关性。材料与方法：这是一项病例对照研究，对90例确诊的精神分裂症患者和90名匹配的健康志愿者作为对照进行研究。从乙二胺四乙酸（EDTA）血样中提取DNA并进行聚合酶链反应（PCR），使用限制性片段长度多态性（RFLP）对NF-κB1基因多态性rs28362691（-94 ATTG插入/缺失）进行研究。结果：我们观察到插入/插入（ins/ins）基因型（78%）在研究人群中更为普遍。缺失/缺失（del/del）和插入/缺失（ins/del）基因型分别在6.7%和14.4%的精神分裂症患者中出现。插入等位基因比缺失等位基因更常见。Pearson相关性分析显示，NF-κB1水平与疾病严重程度之间存在显著正相关，r值为0.471，p值为0.027。结论：我们发现，在精神分裂症患者中，插入等位基因高于缺失等位基因，插入/插入基因型的频率高于缺失/缺失和插入/缺失基因型。插入基因型与精神分裂症患者疾病症状的严重程度之间存在强烈的正相关。
