Atila Alptug, Yilmaz Bilal
Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.
Iran J Pharm Res. 2015 Spring;14(2):443-51.
In this study, simple, fast and reliable cyclic voltammetry (CV), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and differential pulse voltammetry (DPV) methods were developed and validated for determination of bosentan in pharmaceutical preparations. The proposed methods were based on electrochemical oxidation of bosentan at platinum electrode in acetonitrile solution containing 0.1 M TBACIO4. The well-defined oxidation peak was observed at 1.21 V. The calibration curves were linear for bosentan at the concentration range of 5-40 µg/mL for LSV and 5-35 µg/mL for SWV and DPV methods, respectively. Intra- and inter-day precision values for bosentan were less than 4.92, and accuracy (relative error) was better than 6.29%. The mean recovery of bosentan was 100.7% for pharmaceutical preparations. No interference was found from two tablet excipients at the selected assay conditions. Developed methods in this study are accurate, precise and can be easily applied to Tracleer and Diamond tablets as pharmaceutical preparation.
在本研究中，开发并验证了简单、快速且可靠的循环伏安法（CV）、线性扫描伏安法（LSV）、方波伏安法（SWV）和差分脉冲伏安法（DPV），用于测定药物制剂中的波生坦。所提出的方法基于波生坦在含0.1 M TBACIO4的乙腈溶液中的铂电极上进行电化学氧化。在1.21 V处观察到明确的氧化峰。对于LSV方法，波生坦在5 - 40 μg/mL浓度范围内校准曲线呈线性，对于SWV和DPV方法，波生坦在5 - 35 μg/mL浓度范围内校准曲线呈线性。波生坦的日内和日间精密度值均小于4.92，准确度（相对误差）优于6.29%。药物制剂中波生坦的平均回收率为100.7%。在选定的分析条件下，两种片剂辅料均未发现干扰。本研究中开发的方法准确、精密，可轻松应用于作为药物制剂的全可利（Tracleer）和达莫林（Diamond）片剂。
