McClatchy David M, Krishnaswamy Venkataramanan, Kanick Stephen C, Elliott Jonathan T, Wells Wendy A, Barth Richard J, Paulsen Keith D, Pogue Brian W
Dartmouth College, Thayer School of Engineering, 14 Engineering Drive, Hanover, New Hampshire 03755, United States.
Dartmouth College, Department of Pathology, Geisel School of Medicine, 1 Rope Ferry Road, Hanover, New Hampshire 03755, United States.
J Biomed Opt. 2015 Apr;20(4):040504. doi: 10.1117/1.JBO.20.4.040504.
A variety of optical techniques utilizing near-infrared (NIR) light are being proposed for intraoperative breast tumor margin assessment. However, immediately following a lumpectomy excision, the margins are inked, which preserves the orientation of the specimen but prevents optical interrogation of the tissue margins. Here, a workflow is proposed that allows for both NIR optical assessment following full specimen marking using molecular dyes which have negligible absorption and scattering in the NIR. The effect of standard surgical inks in contrast to molecular dyes for an NIR signal is shown. Further, the proposed workflow is demonstrated with full specimen intraoperative imaging on all margins directly after the lumpectomy has been excised and completely marked. This work is an important step in the path to clinical feasibility of intraoperative breast tumor margin assessment using NIR optical methods without having to compromise on the current clinical practice of inking resected specimens for margin orientation.
目前正在提出多种利用近红外（NIR）光的光学技术用于术中乳腺肿瘤边缘评估。然而，在肿块切除术切除后，边缘会立即用墨水标记，这保留了标本的方向，但阻止了对组织边缘进行光学检测。在此，提出了一种工作流程，该流程允许在使用在近红外波段具有可忽略不计的吸收和散射的分子染料对整个标本进行标记后进行近红外光学评估。展示了标准手术墨水与分子染料相比对近红外信号的影响。此外，在肿块切除并完全标记后，直接对所有边缘进行全标本术中成像，展示了所提出的工作流程。这项工作是迈向使用近红外光学方法进行术中乳腺肿瘤边缘评估临床可行性道路上的重要一步，而无需在当前为标记切除标本边缘方向而进行墨水标记的临床实践上做出妥协。
