Tsai Sujune, Thongpooe Preeyanuch, Kuo Fu-Wen, Lin Chiahsin
a Department of Biotechnology , Mingdao University , Peetow , Chang Hua , Taiwan .
b Department of Post Modern Agriculture , Mingdao University , Peetow , Chang Hua , Taiwan .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2512-5. doi: 10.3109/19401736.2015.1036254. Epub 2015 Apr 22.
Given the current threats to coral reefs worldwide, there is an urgent need to develop protocols for the cryopreservation of reef-building corals. However, chilling may alter coral mitochondrial distribution and membrane potential, resulting in reduced ATP production. The aim of this study was to investigate the impacts of chilling on mitochondrial DNA copy number (CN) in oocytes of the hard coral Echinopora sp. Oocytes were exposed to 0.5 M, 1 M or 2 M methanol at 5, 0 or -5 °C for 2, 4, 8 and 16 h. When oocytes were chilled with no cryoprotectant (CPT) or 1 M methanol at 5 or 0 °C, the mtDNA CNs initially increased at hour 2 of incubation, although it decreased significantly over the 16 h of incubation in chilled oocytes at -5 °C. The mtDNA CN increased and picked in 0.5 M methanol at 5 °C and 0 °C at hour 8 of incubation in chilled oocytes indicating that the high mtDNA CN of these oocytes is probably responsible for withstanding high chilling sensitivity. We currently propose that 0.5 M methanol is the optimal CPT for oocytes of Echinopora sp., and potentially other reef corals.
鉴于当前全球珊瑚礁面临的威胁，迫切需要制定造礁珊瑚冷冻保存的方案。然而，低温可能会改变珊瑚线粒体的分布和膜电位，导致ATP生成减少。本研究的目的是调查低温对硬珊瑚棘孔珊瑚（Echinopora sp.）卵母细胞线粒体DNA拷贝数（CN）的影响。将卵母细胞在5℃、0℃或-5℃下分别暴露于0.5M、1M或2M甲醇中2、4、8和16小时。当卵母细胞在5℃或0℃下不使用冷冻保护剂（CPT）或1M甲醇进行低温处理时，线粒体DNA拷贝数在孵育2小时时最初会增加，尽管在-5℃下低温处理的卵母细胞在孵育16小时期间显著下降。在5℃和0℃下，用0.5M甲醇低温处理的卵母细胞在孵育8小时时线粒体DNA拷贝数增加并达到峰值，这表明这些卵母细胞的高线粒体DNA拷贝数可能是其能够耐受高低温敏感性的原因。我们目前提出，0.5M甲醇是棘孔珊瑚卵母细胞以及可能其他造礁珊瑚的最佳冷冻保护剂。
