Heffner L J, Gramates L S, Yuan R W
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Boston, MA.
Biochem Biophys Res Commun. 1989 Nov 30;165(1):299-305. doi: 10.1016/0006-291x(89)91069-3.
Western immunoblots performed during separation of glycosylated prolactin from amniotic fluid revealed that some of the glycosylated prolactin is covalently bound to another protein. Using high performance liquid chromatography and other protein isolation techniques we have demonstrated that a glycosylated prolactin species is linked to immunoglobulin by disulfide bonds in amniotic fluid.
在从羊水分离糖基化催乳素的过程中进行的蛋白质免疫印迹分析显示，部分糖基化催乳素与另一种蛋白质共价结合。我们利用高效液相色谱法和其他蛋白质分离技术证明，羊水内一种糖基化催乳素与免疫球蛋白通过二硫键相连。
