Sowers L C, Eritja R, Chen F M, Khwaja T, Kaplan B E, Goodman M F, Fazakerley G V
Division of Pediatrics, City of Hope National Medical Center, Duarte, CA.
Biochem Biophys Res Commun. 1989 Nov 30;165(1):89-92. doi: 10.1016/0006-291x(89)91037-1.
Transition mutations induced by the base analogue 2-aminopurine arise via the formation of AP.C base pairs during DNA replication. We report here the results of N-15 NMR studies on a duplex oligonucleotide containing N-15 enriched AP and C residues. At high pH (8.6) the AP.C base pair is predominantly wobble. This is the first report on use of a site specifically N-15 enriched oligonucleotide as a probe of aberrant base pairing in DNA.
碱基类似物2-氨基嘌呤诱导的转换突变是在DNA复制过程中通过形成AP·C碱基对产生的。我们在此报告了对含有N-15富集的AP和C残基的双链寡核苷酸进行N-15 NMR研究的结果。在高pH值（8.6）下，AP·C碱基对主要呈摆动状态。这是首次报道使用位点特异性N-15富集的寡核苷酸作为DNA中异常碱基配对的探针。
