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1
Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine.含有涉及2-氨基嘌呤碱基错配的特定DNA寡聚物的合成与性质
Nucleic Acids Res. 1986 Jul 25;14(14):5869-84. doi: 10.1093/nar/14.14.5869.
2
NMR study of the conformation of the 2-aminopurine:cytosine mismatch in DNA.DNA中2-氨基嘌呤与胞嘧啶错配构象的核磁共振研究。
Biochemistry. 1996 Apr 2;35(13):4026-33. doi: 10.1021/bi952657g.
3
NMR studies on an oligodeoxynucleotide containing 2-aminopurine opposite adenine.关于含与腺嘌呤相对的2-氨基嘌呤的寡脱氧核苷酸的核磁共振研究。
Biochemistry. 1987 Sep 8;26(18):5641-6. doi: 10.1021/bi00392a009.
4
Base pairing and mutagenesis: observation of a protonated base pair between 2-aminopurine and cytosine in an oligonucleotide by proton NMR.碱基配对与诱变：通过质子核磁共振观察寡核苷酸中2-氨基嘌呤与胞嘧啶之间的质子化碱基对。
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5434-8. doi: 10.1073/pnas.83.15.5434.
5
Spectroscopic and calorimetric characterizations of DNA duplexes containing 2-aminopurine.含2-氨基嘌呤的DNA双链体的光谱和量热表征
Biochemistry. 1996 Sep 24;35(38):12329-37. doi: 10.1021/bi9614545.
6
An explanation of the induction of mutations by 2-aminopurine from an ab initio molecular orbital study.基于从头算分子轨道研究对2-氨基嘌呤诱导突变的解释。
Mutat Res. 1986 Jan;173(1):49-54. doi: 10.1016/0165-7992(86)90010-2.
7
Evidence of 2-aminopurine-cytosine base mispairs involving two hydrogen bonds.涉及两个氢键的2-氨基嘌呤-胞嘧啶碱基错配的证据。
J Biol Chem. 1983 Nov 10;258(21):12842-6.
8
Characterization of the high pH wobble structure of the 2-aminopurine.cytosine mismatch by N-15 NMR spectroscopy.通过N-15核磁共振光谱法对2-氨基嘌呤-胞嘧啶错配的高pH摆动结构进行表征。
Biochem Biophys Res Commun. 1989 Nov 30;165(1):89-92. doi: 10.1016/0006-291x(89)91037-1.
9
The interaction of DNA duplexes containing 2-aminopurine with restriction endonucleases EcoRII and SsoII.含2-氨基嘌呤的DNA双链体与限制性内切酶EcoRII和SsoII的相互作用。
Nucleic Acids Res. 1995 Jun 25;23(12):2192-7. doi: 10.1093/nar/23.12.2192.
10
Base pair opening dynamics of a 2-aminopurine substituted Eco RI restriction sequence and its unsubstituted counterpart in oligonucleotides.2-氨基嘌呤取代的Eco RI限制序列及其在寡核苷酸中的未取代对应序列的碱基对打开动力学。
Nucleic Acids Res. 1987 Nov 11;15(21):9011-25. doi: 10.1093/nar/15.21.9011.

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J Fluoresc. 1992 Dec;2(4):209-16. doi: 10.1007/BF00865278.
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Ambivalent incorporation of the fluorescent cytosine analogues tC and tCo by human DNA polymerase alpha and Klenow fragment.人DNA聚合酶α和克列诺片段对荧光胞嘧啶类似物tC和tCo的矛盾掺入
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Spectroscopic studies of position-specific DNA "breathing" fluctuations at replication forks and primer-template junctions.复制叉和引物-模板连接处特定位置DNA“呼吸”波动的光谱研究。
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本文引用的文献

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Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codons.细菌视紫红质中特定氨基酸的取代：用含有改变了的密码子的合成 DNA 片段替换结构基因中的限制酶片段。
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2
THE DIFFERENCE BETWEEN SPONTANEOUS AND BASE-ANALOGUE INDUCED MUTATIONS OF PHAGE T4.噬菌体T4自发突变与碱基类似物诱导突变之间的差异
Proc Natl Acad Sci U S A. 1959 Apr;45(4):622-33. doi: 10.1073/pnas.45.4.622.
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Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA.5-氟尿嘧啶对突变表型的逆转：一种研究信使核糖核酸中核苷酸序列的方法。
Proc Natl Acad Sci U S A. 1962 Apr 15;48(4):532-46. doi: 10.1073/pnas.48.4.532.
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Mutation as an error in base pairing.作为碱基配对错误的突变。
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5
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
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Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
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2-Aminopurine.2-氨基嘌呤
Mutat Res. 1980 Jan;75(1):1-47. doi: 10.1016/0165-1110(80)90026-3.
8
On the molecular basis of transition mutations: frequencies of forming 2-aminopurine.cytosine and adenine.cytosine base mispairs in vitro.关于转换突变的分子基础：体外形成2-氨基嘌呤·胞嘧啶和腺嘌呤·胞嘧啶碱基错配的频率
Proc Natl Acad Sci U S A. 1981 May;78(5):2864-8. doi: 10.1073/pnas.78.5.2864.
9
Evidence of 2-aminopurine-cytosine base mispairs involving two hydrogen bonds.涉及两个氢键的2-氨基嘌呤-胞嘧啶碱基错配的证据。
J Biol Chem. 1983 Nov 10;258(21):12842-6.
10
Dialkylformamidines: depurination resistant N6-protecting group for deoxyadenosine.二烷基甲脒：用于脱氧腺苷的抗脱嘌呤N6保护基团。
Nucleic Acids Res. 1983 Nov 25;11(22):8031-6. doi: 10.1093/nar/11.22.8031.

含有涉及2-氨基嘌呤碱基错配的特定DNA寡聚物的合成与性质

Synthesis and properties of defined DNA oligomers containing base mispairs involving 2-aminopurine.

作者信息

Eritja R, Kaplan B E, Mhaskar D, Sowers L C, Petruska J, Goodman M F

出版信息

Nucleic Acids Res. 1986 Jul 25;14(14):5869-84. doi: 10.1093/nar/14.14.5869.

DOI:10.1093/nar/14.14.5869
PMID:3737416
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311597/
Abstract

DNA heptamers containing the mutagenic base analogue 2-aminopurine (AP) have been chemically synthesized and physically characterized. We report on the relative stabilities of base pairs between AP and each of the common DNA bases, as determined from heptamer duplex melts at 275 and 330 nm. Base pairs are ranked in order of decreasing stability: AP.T greater than AP.A greater than AP.C greater than AP.G. It is of interest that AP.A is more stable than AP.C even though DNA polymerase strongly favors the formation of AP.C over AP.A base pairs. Comparisons of melting profiles at 330 nm and 275 nm indicate that AP.T, AP.A, and AP.C base pairs are annealed in heptamer duplexes and melt 2-3 degrees prior to surrounding base pairs, whereas AP.G appears not to be annealed.

摘要

含有诱变碱基类似物2-氨基嘌呤（AP）的DNA七聚体已被化学合成并进行了物理表征。我们报告了从七聚体双链体在275和330nm处的熔解情况确定的AP与每个常见DNA碱基之间碱基对的相对稳定性。碱基对按稳定性降低的顺序排列：AP.T大于AP.A大于AP.C大于AP.G。有趣的是，尽管DNA聚合酶强烈倾向于形成AP.C而非AP.A碱基对，但AP.A比AP.C更稳定。330nm和275nm处熔解曲线的比较表明，AP.T、AP.A和AP.C碱基对在七聚体双链体中退火，并且在周围碱基对之前2-3度熔解，而AP.G似乎没有退火。