Li Li, Chen Ye-ye, Li Shan-qing, Huang Cheng, Qin Ying-zhi
Department of Thoracic Surgery, Peking Union Medical College Hospital, Beijing, China (mainland).
Med Sci Monit. 2015 Apr 23;21:1155-61. doi: 10.12659/MSM.892940.
Altered miR-148/152 family expression contributes to human carcinogenesis. This study was designed to detect the potential for using miR-148/152 family as biomarkers for NSCLC patients.
MATERIAL/METHODS: The relative expression levels of miR-148/152 family (miR-148a, miR-148b, and miR-152) in serum of 36 non-small-cell lung carcinoma (NSCLC) patients, 20 patients with benign pulmonary diseases (BPD), and 10 healthy individuals were assessed by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR).
The expression of all three miRNAs were significantly lower in the serum of NSCLC than that of BPD and healthy controls (all p<0.01), and their expression levels were strongly correlated with each other (r=0.781, 0.720, and 0.645, respectively). Downregulation of miR-148/152 family was found to be corrected with more aggressive tumors. The area under the receiver operating characteristic curves (AUCs) for miR-148a, miR-148b, and miR-152 discriminating NSCLC from BPD were 0.775, 0.725, and 0.774, respectively, all higher than that of CEA (0.506). Combining the three miRNAs increased the discrimination performance, yielding an AUC of 0.789 (95% confidence interval, 0.643 to 0.895), with a sensitivity of 72.2% and a specificity of 90.0%.
The results of present study suggest that the expression levels of circulating miR-148/152 family may serve as biomarkers for NSCLC.
miR-148/152家族表达改变与人类致癌作用有关。本研究旨在检测将miR-148/152家族用作非小细胞肺癌（NSCLC）患者生物标志物的可能性。
材料/方法：采用实时逆转录定量聚合酶链反应（RT-qPCR）评估36例非小细胞肺癌（NSCLC）患者、20例良性肺疾病（BPD）患者和10名健康个体血清中miR-148/152家族（miR-148a、miR-148b和miR-152）的相对表达水平。
NSCLC患者血清中所有三种miRNA的表达均显著低于BPD患者和健康对照（均p<0.01），且它们的表达水平彼此高度相关（分别为r=0.781、0.720和0.645）。发现miR-148/152家族的下调与更具侵袭性的肿瘤相关。miR-148a、miR-148b和miR-152区分NSCLC与BPD的受试者工作特征曲线（AUC）下面积分别为0.775、0.725和0.774，均高于癌胚抗原（CEA）的0.506。联合这三种miRNA可提高鉴别性能，AUC为0.789（95%置信区间，0.643至0.895），敏感性为72.2%，特异性为90.0%。
本研究结果表明，循环miR-148/152家族的表达水平可能是非小细胞肺癌的生物标志物。
