Šćepović Tanja, Jokić Goran, Esther Alexandra, Kataranovski Dragan, Vukša Petar, Đedović Suzana, Vukša Marina
Institute of Pesticides and Environmental Protection, Zemun, Belgrade, Serbia.
Vertebrate Research, Institute for Plant Protection in Horticulture and Forestry, Julius Kühn Institute, Federal Research Institute for Cultivated Plants, Münster, Germany.
Pest Manag Sci. 2016 Mar;72(3):574-9. doi: 10.1002/ps.4027. Epub 2015 May 13.
After reports of management problems in practice, a survey was conducted to determine the presence of bromadiolone-resistant animals in different house mouse (Mus musculus L.) populations in Serbia. A 21 day no-choice feeding test was carried out to examine the resistance of house mice to bromadiolone. Eighty house mice collected from four locations (ten males and ten females per location) were tested for bromadiolone tolerance. Surviving animals and their F1 offspring were screened for mutations. The influence of VKOR variant, zygosity and sex on bromadiolone tolerance was analysed.
Bait intake and changes in body weight revealed different animal responses regarding susceptibility or resistance. Leu128Ser, Tyr139Cys and a new Ala21Thr polymorphism were detected in wild-born survivors and their F1 generation. However, not every individual with the polymorphisms Leu128Ser and Tyr139Cys survived the feeding test. VKOR variants and sex caused variations in bromadiolone tolerance.
For the first time it was shown that the VKOR variant, along with sex, is responsible for bromadiolone tolerance in house mice. Other factors influencing bromadiolone tolerance, including sex-specific factors, cannot be excluded. The tolerance levels of VKOR variants should be determined in further studies in order to evaluate the effectiveness of bromadiolone in sustainable management.
在有实践中管理问题的报告之后，开展了一项调查以确定塞尔维亚不同家鼠（小家鼠）种群中抗溴敌隆动物的存在情况。进行了一项为期21天的无选择摄食试验，以检测家鼠对溴敌隆的抗性。从四个地点收集了80只家鼠（每个地点10只雄性和10只雌性），检测它们对溴敌隆的耐受性。对存活的动物及其F1后代进行突变筛查。分析了维生素K环氧化物还原酶（VKOR）变体、纯合性和性别对溴敌隆耐受性的影响。
诱饵摄入量和体重变化揭示了动物在易感性或抗性方面的不同反应。在野生出生的存活者及其F1代中检测到Leu128Ser、Tyr139Cys和一种新的Ala21Thr多态性。然而，并非每个具有Leu128Ser和Tyr139Cys多态性的个体都在摄食试验中存活下来。VKOR变体和性别导致了溴敌隆耐受性的差异。
首次表明VKOR变体与性别一起决定了家鼠对溴敌隆的耐受性。不能排除其他影响溴敌隆耐受性的因素，包括性别特异性因素。为了评估溴敌隆在可持续管理中的有效性，应在进一步研究中确定VKOR变体的耐受水平。
