Frankova Marcela, Starostova Zuzana, Aulicky Radek, Stejskal Vaclav
Czech Agrifood Research Center, Drnovska 507/73, CZ-16100, Prague 6, Czech Republic.
Department of Zoology, Faculty of Science, Charles University, Vinicna 7, CZ-12800, Prague 2, Czech Republic.
Sci Rep. 2025 Jan 11;15(1):1701. doi: 10.1038/s41598-025-85447-8.
Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast to studies on Western European house mice (Mus musculus domesticus), few resistance studies have focused on Eastern European house mice (M. musculus musculus), which have a western distribution boundary in the Czech Republic. This study newly analysed the VKORC1 gene in M. m. musculus field populations from Czech farms and grain stores and identified a nonsynonymous mutation Tyr139Phe. This mutation was common throughout the Czech Republic and was present in 80.2% of the 86 individuals sampled. Additionally, all individuals exhibited a genotype with three synonymous mutations specific to the subspecies M. m. musculus. The functional (mortality-survival) response of the Tyr139Phe mutation was validated in a laboratory choice feeding test using bromadiolone-based bait, where all resistant homozygous individuals survived, while all susceptible mice died, with a mean survival of 6.9 days.
尽管抗凝血灭鼠剂在控制家栖害鼠的诱饵中广泛使用，但由于二次中毒和抗药性增加，其应用存在问题。与对西欧家鼠（小家鼠）的研究相比，很少有抗药性研究关注东欧家鼠（小家鼠指名亚种），其在捷克共和国有一个西部分布边界。本研究新分析了来自捷克农场和粮库的小家鼠指名亚种野外种群中的VKORC1基因，并鉴定出一个非同义突变Tyr139Phe。该突变在捷克共和国各地都很常见，在抽样的86个个体中有80.2%存在该突变。此外，所有个体都表现出一种具有三个特定于小家鼠指名亚种的同义突变的基因型。在使用溴敌隆诱饵的实验室选择摄食试验中验证了Tyr139Phe突变的功能（死亡率-存活率）反应，其中所有抗性纯合个体存活，而所有易感小鼠死亡，平均存活6.9天。
