†Laboratoire d'Electrochimie Moléculaire, Université Paris Diderot, Sorbonne Paris Cité, Unité Mixte de Recherche Université, CNRS No 7591, Bâtiment Lavoisier, 15 rue Jean-Antoine de Baïf, 75205 Cedex 13 Paris, France.
‡UMR 1332 Biologie du Fruit et Pathologie, INRA-Université Bordeaux 2, 71 av. Edouard Bourlaux, 20032-33882 Cedex Villenave d'Ornon, France.
ACS Nano. 2015 May 26;9(5):4911-24. doi: 10.1021/acsnano.5b00952. Epub 2015 Apr 29.
We show herein that electrochemical atomic force microscopy (AFM-SECM), operated in molecule touching (Mt) mode and combined with redox immunomarking, enables the in situ mapping of the distribution of proteins on individual virus particles and makes localization of individual viral proteins possible. Acquisition of a topography image allows isolated virus particles to be identified and structurally characterized, while simultaneous acquisition of a current image allows the sought after protein, marked by redox antibodies, to be selectively located. We concomitantly show that Mt/AFM-SECM, due to its single-particle resolution, can also uniquely reveal the way redox functionalization endowed to viral particles is distributed both statistically among the viruses and spatially over individual virus particles. This possibility makes Mt/AFM-SECM a unique tool for viral nanotechnology.
我们在此展示，电化学原子力显微镜（AFM-SECM）在分子接触（Mt）模式下运行，并结合氧化还原免疫标记，能够对单个病毒颗粒上蛋白质的分布进行原位映射，并实现对单个病毒蛋白的定位。形貌图像的获取可识别和结构表征分离的病毒颗粒，而同时获取电流图像可选择性定位被氧化还原抗体标记的目标蛋白。我们同时表明，由于 Mt/AFM-SECM 的单颗粒分辨率，它还可以独特地揭示赋予病毒颗粒的氧化还原功能化在病毒之间的统计学分布方式，以及在单个病毒颗粒上的空间分布方式。这种可能性使 Mt/AFM-SECM 成为病毒纳米技术的独特工具。
