Yu Yuanman, Chen Jie, Chen Rui, Cao Lingyan, Tang Wei, Lin Dan, Wang Jing, Liu Changsheng
†The State Key Laboratory of Bioreactor Engineering, ‡Key Laboratory for Ultrafine Materials of Ministry of Education, §Engineering Research Center for Biomedical Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
ACS Appl Mater Interfaces. 2015 May 13;7(18):9982-90. doi: 10.1021/acsami.5b02324. Epub 2015 May 4.
Rapid and controlled vascularization within scaffolds remains one of the key limitations in tissue engineering applications. This study describes the fabrication and characterization of 2-N,6-O-sulfated chitosan (26SCS)-coated hierarchical scaffold composed of poly(lactic-co-glycolic acid) (PLGA) microspheres, as a desirable vehicle for vascular endothelial growth factor (VEGF) delivery and consequent angiogenic boosting in vitro. Owing to the hierarchical porous structure and high affinity between VEGF and 26SCS, the 26SCS-modified PLGA (S-PLGA) scaffold possesses excellent entrapment and sustained release of VEGF. Using human umbilical vein endothelial cells (HUVECs) as a cell model, the VEGF-loaded S-PLGA scaffold shows desirable cell viability and attachment. The bioactivity of released VEGF is validated by intracellular nitric oxide secretion and capillary tube formation, demonstrating the improved capacity of VEGF-mediated pro-angiogenesis ascribed to 26SCS incorporation. Such a strategy will afford an effective method to prepare a scaffold with promoted angiogenesis.
支架内快速且可控的血管化仍然是组织工程应用中的关键限制之一。本研究描述了由聚乳酸 - 乙醇酸共聚物（PLGA）微球组成的2 - N，6 - O - 硫酸化壳聚糖（26SCS）涂层分级支架的制备与表征，该支架是用于血管内皮生长因子（VEGF）递送及随后在体外促进血管生成的理想载体。由于分级多孔结构以及VEGF与26SCS之间的高亲和力，26SCS修饰的PLGA（S - PLGA）支架具有优异的VEGF包封和缓释性能。以人脐静脉内皮细胞（HUVECs）作为细胞模型，负载VEGF的S - PLGA支架表现出理想的细胞活力和细胞附着。释放的VEGF的生物活性通过细胞内一氧化氮分泌和毛细管形成得到验证，证明了由于掺入26SCS而使VEGF介导的促血管生成能力得到改善。这种策略将提供一种制备具有促进血管生成的支架的有效方法。
