Krasinskaia I P, Litvinov I S, Zakharov S D, Bakeeva L E, Iaguzhinskiĭ L S
Biokhimiia. 1989 Sep;54(9):1550-6.
Low (120 mosM) tonicity of incubation media of mitochondria was found to be associated with anomalous phase transition at 19--26 degrees C. A rise in temperature caused a decrease in the pyrene excitation in border lipids of the mitochondrial membrane. Within this temperature range the quenching of intrinsic protein fluorescence by pyrene was sharply decreased. It may be inferred from these data that at 100mosM tonicity and temperatures below 19 degrees C, mitochondrial membrane proteins are in an aggregated state. At temperatures above phase transition protein deaggregation takes place. It was shown that a decrease in tonicity from 300 to 120 mosM at 15 degrees C or a rise in temperature from 15 degrees to 37 degrees C at 300 mosM tonicity increased the phosphorylation of the 52 kDa mitochondrial protein. It was assumed that swelling of mitochondria in hypotonic media simulates one of the steps of the hormone-induced signal transfer in mitochondria in vivo.
研究发现，线粒体孵育介质的低渗（120 mosM）状态与19-26摄氏度下的异常相变有关。温度升高导致线粒体膜边界脂质中芘的激发减少。在此温度范围内，芘对内在蛋白荧光的猝灭急剧下降。从这些数据可以推断，在100 mosM的渗透压和低于19摄氏度的温度下，线粒体膜蛋白处于聚集状态。在高于相变温度时，蛋白发生解聚。结果表明，在15摄氏度下渗透压从300降至120 mosM，或在300 mosM渗透压下温度从15摄氏度升至37摄氏度，都会增加52 kDa线粒体蛋白的磷酸化。据推测，低渗介质中线粒体的肿胀模拟了体内激素诱导的线粒体信号传递步骤之一。
