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[温度变化后皮质类固醇对线粒体中苯胺萘磺酸盐荧光的影响]

[Effect of corticosteroids on the fluorescence of aniline naphthalenesulfonate in mitochondria after changes in temperature].

作者信息

Vol'skiĭ G G, Zoidze S D, Burgova M P

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1987(1):30-4.

PMID:3828407
Abstract

The influence of corticosteroid hormones and cholesterol on the anilinonaphthalenesulfonate (ANS-) fluorescence in the rat liver mitochondria at different temperatures has been studied. The fluorescence intensity has been found to decrease by warming and increase by cooling the mitochondrial suspension. The steroids studied in proportion to their hydrophobic nature decreased the ANS- fluorescence at 25 degrees C and increased it at 5 degrees C, i.e. at higher and lower temperatures of the phase transition of the membrane lipids. It has been concluded that steroid hormones influence the fluidity of the mitochondrial membranes.

摘要

研究了皮质类固醇激素和胆固醇在不同温度下对大鼠肝线粒体中苯胺萘磺酸盐(ANS-)荧光的影响。发现通过加热线粒体悬浮液,荧光强度降低;通过冷却线粒体悬浮液,荧光强度增加。所研究的类固醇根据其疏水性质,在25℃时降低ANS-荧光,在5℃时增加ANS-荧光,即在膜脂相变的较高和较低温度下。得出的结论是,类固醇激素影响线粒体膜的流动性。

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