Sled' V D, Zinich V N, Kotliar A B
Biokhimiia. 1989 Sep;54(9):1571-5.
The mechanism of ubiquinone homologs reduction by different preparations of mitochondrial NADH dehydrogenase: complex I within submitochondrial particles, isolated NADH-ubiquinone oxidoreductase and soluble low molecular weight NADH dehydrogenase, has been investigated. It has been shown that NADH oxidation via the rotenone-insensitive reaction is associated with one-electron reduction of low molecular weight ubiquinone homologs (Q0, Q1, Q2) to semiquinone with subsequent fast oxidation of the latter by atmospheric oxygen to form a superoxide radical. The two-electron ubiquinone reduction to quinol in the rotenone-sensitive reaction is unaccompanied by the semiquinone release from the enzyme active center into the surrounding solution.
研究了线粒体质NADH脱氢酶的不同制剂（亚线粒体颗粒中的复合物I、分离的NADH-泛醌氧化还原酶和可溶性低分子量NADH脱氢酶）还原泛醌同系物的机制。结果表明，通过鱼藤酮不敏感反应进行的NADH氧化与低分子量泛醌同系物（Q0、Q1、Q2）单电子还原为半醌有关，随后半醌被大气中的氧气快速氧化形成超氧自由基。在鱼藤酮敏感反应中，泛醌双电子还原为醌醇的过程中，半醌不会从酶活性中心释放到周围溶液中。
