Ayan M, Tas U, Sogut E, Caylı S, Kaya H, Esen M, Erdemir F, Uysal M
Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.
Andrologia. 2016 Mar;48(2):143-51. doi: 10.1111/and.12424. Epub 2015 Apr 23.
We aimed to determine the protective effects of thymoquinone (TQ), against ischaemia-reperfusion (I/R) injury in the testis tissue of rats. Twenty-seven male Wistar albino rats were randomly divided into three equal groups as follows: Group I, sham group; Group II, torsion group; and Group III, torsion + thymoquinone group. The ischaemia period was 2 h, and orchiectomy was performed after 30 min of detorsion. Testis tissue sections were analysed with the terminal transferase mediated dUTP-nick end labelling (TUNEL) assay to determine in situ apoptotic DNA fragmentation. Additionally, Caspase 3 and Bax proteins were analysed immunohistochemically. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) activity levels in the testis tissue were also measured. The superoxide dismutase activity and malondialdehyde levels in the torsion group were significantly higher than those of the sham group (P < 0.05). Thymoquinone administration significantly reduced these levels. Torsion significantly increased active-Caspase 3 and Bax expression, which was decreased by thymoquinone. The apoptotic index of the torsion group was significantly higher than that of the control group. However, thymoquinone significantly reduced the apoptotic index (P < 0.05). Our results indicate that thymoquinone plays a protective role in oxidative stress induced ischaemia-reperfusion in the testis tissue of rats.
我们旨在确定百里醌（TQ）对大鼠睾丸组织缺血再灌注（I/R）损伤的保护作用。将27只雄性Wistar白化大鼠随机分为三组，每组数量相等，如下：第一组，假手术组；第二组，扭转组；第三组，扭转+百里醌组。缺血期为2小时，扭转解除30分钟后进行睾丸切除术。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法分析睾丸组织切片，以确定原位凋亡DNA片段化情况。此外，采用免疫组织化学法分析半胱天冬酶3（Caspase 3）和Bax蛋白。还测定了睾丸组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的活性水平。扭转组的超氧化物歧化酶活性和丙二醛水平显著高于假手术组（P<0.05）。给予百里醌可显著降低这些水平。扭转显著增加了活性Caspase 3和Bax的表达，而百里醌可使其降低。扭转组的凋亡指数显著高于对照组。然而，百里醌显著降低了凋亡指数（P<0.05）。我们的结果表明，百里醌对大鼠睾丸组织氧化应激诱导的缺血再灌注具有保护作用。
