Yu Zhou, Karkaria Tishtar, Espina Marianela, Hunjun Manjeet, Surendran Abera, Luu Tina, Telychko Julia, Yang Yan-Ping
Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
Bioprocess Research and Development, Sanofi Pasteur, Toronto, Canada M2R 3T4.
J Biotechnol. 2015 Jul 20;206:38-41. doi: 10.1016/j.jbiotec.2015.04.003. Epub 2015 Apr 20.
Membrane chromatography is increasingly used for protein purification in the biopharmaceutical industry. Membrane adsorbers are often pre-assembled by manufacturers as ready-to-use modules. In large-scale protein manufacturing settings, the use of multiple membrane modules for a single batch is often required due to the large quantity of feed material. The question as to how multiple modules can be connected to achieve optimum separation and productivity has been previously approached using model proteins and mass transport theories. In this study, we compare the performance of multiple membrane modules in series and in parallel in the production of a protein antigen. Series connection was shown to provide superior separation compared to parallel connection in the context of competitive adsorption.
