Demmer W, Nussbaumer D
Sartorius AG, Göttingen, Germany.
J Chromatogr A. 1999 Aug 6;852(1):73-81. doi: 10.1016/s0021-9673(99)00603-2.
A new large-scale membrane adsorber system for rapid separation of biomolecules based on a hollow cylindrical module geometry in unitized construction and combined parallel and serial connection of modules is presented. The effectiveness of the concept is demonstrated by breakthrough curves on a 21-m2 pilot plant with hemoglobin as a model substance. Scale-up to the order of 100 m2 and above is easily performed by combining modules in parallel for increasing capacity, and in series for optimizing performance. An estimation of productivity based on these data is presented. Preliminary results of separations by linear salt gradient elution are also given.
本文介绍了一种新型大规模膜吸附器系统，该系统基于单元化结构的空心圆柱形模块几何形状，以及模块的并联和串联连接，用于快速分离生物分子。以血红蛋白为模型物质，通过21平方米中试装置的穿透曲线证明了该概念的有效性。通过并联模块以增加容量，串联模块以优化性能，可以轻松地扩大到100平方米及以上规模。基于这些数据给出了生产率的估计值。还给出了线性盐梯度洗脱分离的初步结果。
