Wang Zhao-Jun, Deng Chun-Yu, Kuang Su-Juan, Nong Li-Dan, Zhang Guang-Yan, Ma Jue, Cui Jian-Xiu
1Southern Medical University, Guangzhou 510515, China; 3Department of Anesthesiology, Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou 510080, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Apr;35(4):540-3.
To determine the effect of resveratrol on constrictions of isolated human intrapulmonary arteries and its mechanisms.
Intrapulmonary arteries (1-1.5 mm in diameter) were dissected and cut into rings (1.8-2.0 mm in length) under microscope, and were then mounted in a Multi Myograph system. The rings were stimulated with 100 nmol/L U46619, 30 nmol/L endothelin-1, or 60 mmol/L KCl to produce sustained contraction of the intrapulmonary arteries, after which resveratrol was applied cumulatively. Endothelium denudation, L-NAME and indomethecin were used to investigate the effect of resveratrol on constrictions of the isolated arteries, suing DMSO as the control.
Resveratrol induced concentration-dependent relaxations in endothelium-intact rings that contracted in response to stimulations with U46619, ET-1 and KCl, with pD2 of 3.82±0.20, 3.84±0.57, and 3.68±0.27, Emax of (99.58±0.83)%, 100%, and (99.65±0.98)%, respectively. Treatment of the arterial rings with the eNOS inhibitor L-NAME, but not with indomethecin or endothelium denudation, obviously affected the relaxant effects of resveratrol.
Resveratrol can concentration-dependently produce relaxant effect on human intrapulmonary arteries independent of the endothelium possibly by promoting synthesis and release of NO.
确定白藜芦醇对人离体肺内动脉收缩的影响及其机制。
在显微镜下解剖直径为1 - 1.5mm的肺内动脉并切成长度为1.8 - 2.0mm的血管环，然后安装在多功能肌张力测定系统中。用100nmol/L的U46619、30nmol/L的内皮素 - 1或60mmol/L的KCl刺激血管环以产生肺内动脉的持续收缩，之后累积应用白藜芦醇。采用内皮剥脱、L - 精氨酸甲酯（L - NAME）和吲哚美辛来研究白藜芦醇对离体动脉收缩的影响，以二甲基亚砜（DMSO）作为对照。
白藜芦醇在因U46619、ET - 1和KCl刺激而收缩的内皮完整血管环中诱导浓度依赖性舒张，其pD2分别为3.82±0.20、3.84±0.57和3.68±0.27，最大效应（Emax）分别为(99.58±0.83)%、100%和(99.65±0.98)%。用内皮型一氧化氮合酶（eNOS）抑制剂L - NAME处理动脉环，而非用吲哚美辛或内皮剥脱处理，明显影响白藜芦醇的舒张作用。
白藜芦醇可通过促进一氧化氮（NO）的合成和释放，浓度依赖性地对人肺内动脉产生舒张作用，且与内皮无关。
