Lin Hai-Ling, Li Guo-Jian, Wu Ji-Zhou
Endoscopic Clinic, Tumor Hospital Affiliated to Guangxi Medical University, Department of Infectious Diseases, Nanning 530000, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Apr;35(4):573-7.
To explore the effect of aluminum phosphate gel and Kangfuxin on esophageal pathology and expressions of interleukin-8 (IL-8) and prostaglandin E2 (PGE2) in rats with reflux esophagitis and explore the possible mechanisms.
Sixty SD rats were randomized into aluminum phosphate gel group (n=10), Kangfuxin group (n=10), aluminum phosphate gel+Kangfuxin group (n=10), model group (n=20), and control group (n=10). Except for those in the control group, all the rats were subjected to infusion of diluted lysolecithin with hydrochloric acid in the esophagus for 14 days. Ten rats in the model group and those in the control group were sacrificed to examine the pathological changes and contents of IL-8 and PGE2 in the esophagus using optical and electron microscopes and radioimmunoassay. The next day the rest rats were given corresponding treatments (saline in model group) administered into the esophagus on a daily basis for 14 days, after which esophageal pathologies and IL-8 and PGE2 contents were examined.
The model rats showed obvious esophageal pathologies including inflammatory cell infiltration, vacuolar degeneration of the epithelial cells, esophageal erosion and even ulceration, with severe detachment of the epithelial cells. The rats in all the intervention groups showed lessened esophageal pathologies and lowered esophageal IL-8 and PGE2 contents compared with those in the model group. Esophageal mucosal injury index and IL-8 and PGE2 contents were all significantly lower in rats receiving combined treatment with aluminum phosphate and Kangfuxin than in those receiving either of the treatments (P<0.05).
Both Kangfuxin and aluminum phosphate gel are effective in the treatment for reflux esophagitis induced by lysolecithin and hydrochloric acid, and their therapeutic effects are achieved possibly by reducing IL-8 and PGE2 levels in the esophagus.
探讨磷酸铝凝胶和康复新对反流性食管炎大鼠食管病理变化及白细胞介素-8（IL-8）和前列腺素E2（PGE2）表达的影响，并探讨其可能机制。
将60只SD大鼠随机分为磷酸铝凝胶组（n = 10）、康复新组（n = 10）、磷酸铝凝胶+康复新组（n = 10）、模型组（n = 20）和对照组（n = 10）。除对照组外，其余大鼠均经食管灌注稀释的卵磷脂加盐酸，持续14天。处死模型组10只大鼠和对照组大鼠，用光镜、电镜及放射免疫法检测食管病理变化及IL-8和PGE2含量。次日，其余大鼠每日经食管给予相应处理（模型组给予生理盐水），共14天，之后检测食管病理及IL-8和PGE2含量。
模型大鼠食管出现明显病理变化，包括炎症细胞浸润、上皮细胞空泡变性、食管糜烂甚至溃疡，上皮细胞严重脱落。与模型组相比，各干预组大鼠食管病理变化减轻，食管IL-8和PGE2含量降低。磷酸铝与康复新联合治疗的大鼠食管黏膜损伤指数及IL-8和PGE2含量均显著低于单一治疗组（P<0.05）。
康复新和磷酸铝凝胶对卵磷脂和盐酸诱导的反流性食管炎均有治疗作用，其治疗效果可能是通过降低食管中IL-8和PGE2水平实现的。
