Cui Lishan, Xu Songbai, Song Zhengmao, Zhao Gang, Liu Xiaoqian, Song Yuwen
Department of Neurosurgery, The First Affiliated Hospital of Jilin University, Changchun 130021, China Department of Neurosurgery, The Fifth Hospital of Xiamen, Xiamen 361101, China.
Department of Neurosurgery, The First Affiliated Hospital of Jilin University, Changchun 130021, China.
Acta Biochim Biophys Sin (Shanghai). 2015 Jun;47(6):414-21. doi: 10.1093/abbs/gmv026. Epub 2015 Apr 23.
Glioma which has strong proliferation and angiogenesis ability is the most common and malignant primary tumor in central nervous system. Pituitary tumor transforming gene (PTTG) is found in pituitary tumor, and plays important role in cell proliferation, cell cycle, cell apoptosis, and angiogenesis. However, the role of PTTG in glioma is still incompletely investigated. Here, we explored the correlation between PTTG and glioma grade, as well as micro-vessel density (MVD). In addition, siRNA was used to silence PTTG expression in glioma cell lines including U87MG, U251, and SHG44. Cell proliferation, apoptosis, invasion, and angiogenesis were studied both in vitro and in vivo. Our results demonstrated that PTTG expression was significantly up-regulated in glioma, and had positive correlation with glioma grade and MVD. Silencing of PTTG inhibited glioma cell proliferation, migration/invasion, and angiogenesis, induced cell apoptosis, suppressed cell invasion, and arrested cell cycle at G0/G1 stage. Silencing of PTTG could also inhibit tumor growth, invasion, and angiogenesis in vivo. Our data indicated that PTTG might be a potential target for glioma treatment.
胶质瘤是中枢神经系统中最常见的恶性原发性肿瘤，具有很强的增殖和血管生成能力。垂体肿瘤转化基因（PTTG）在垂体肿瘤中被发现，在细胞增殖、细胞周期、细胞凋亡和血管生成中发挥重要作用。然而，PTTG在胶质瘤中的作用仍未得到充分研究。在此，我们探讨了PTTG与胶质瘤分级以及微血管密度（MVD）之间的相关性。此外，我们使用小干扰RNA（siRNA）沉默包括U87MG、U251和SHG44在内的胶质瘤细胞系中的PTTG表达。在体外和体内研究了细胞增殖、凋亡、侵袭和血管生成情况。我们的结果表明，PTTG在胶质瘤中表达显著上调，且与胶质瘤分级和MVD呈正相关。沉默PTTG可抑制胶质瘤细胞增殖、迁移/侵袭和血管生成，诱导细胞凋亡，抑制细胞侵袭，并使细胞周期停滞在G0/G1期。沉默PTTG还可在体内抑制肿瘤生长、侵袭和血管生成。我们的数据表明，PTTG可能是胶质瘤治疗的一个潜在靶点。
