Frederiksen Rikki F, Leisner Jørgen J
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870 Frederiksberg C, Denmark.
Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 15, 1870 Frederiksberg C, Denmark
FEMS Microbiol Lett. 2015 May;362(10). doi: 10.1093/femsle/fnv067. Epub 2015 Apr 23.
Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intracellular survival in Dictyostelium discoideum, and for Salmonella, also infection of mammalian cell lines, and a mouse model. The Salmonella chitinase did not contribute significantly to infection of D. discoideum, mammalian cell lines or mice. However, survival in D. discoideum was clearly reduced for Listeria mutants deficient of ChiB (8-fold) or deficient of both ChiA and ChiB (22-fold). Our findings suggest that chitinases from the two species play different roles in virulence.
一些细菌病原体在宿主感染期间产生几丁质酶作为毒力因子。此类酶在非几丁质宿主中的分子靶点仍不确定。我们研究了单核细胞增生李斯特菌EGD-e和肠炎沙门氏菌血清型鼠伤寒LT2的几丁质酶对在盘基网柄菌中细胞内存活的重要性，对于沙门氏菌，还研究了其对哺乳动物细胞系感染以及对小鼠模型感染的重要性。沙门氏菌几丁质酶对盘基网柄菌、哺乳动物细胞系或小鼠的感染没有显著贡献。然而，对于缺乏ChiB的李斯特菌突变体（8倍）或同时缺乏ChiA和ChiB的突变体（22倍），其在盘基网柄菌中的存活率明显降低。我们的研究结果表明，这两个物种的几丁质酶在毒力方面发挥不同作用。
