Department of Anesthesiology and Medical Crisis Management, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601 Japan.
Department of Experimental Pathology and Tumor Biology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601 Japan.
J Intensive Care. 2014 Mar 6;2(1):21. doi: 10.1186/2052-0492-2-21. eCollection 2014.
Infusion fluids may be contaminated with different types of particulates that are a potential health hazard. Particulates larger than microvessels may cause an embolism by mechanical blockage and inflammation; however, it has been reported that particulates smaller than capillary diameter are relatively safe. Against such a background, one report showed that polystyrene beads smaller than capillary diameter decreased tissue perfusion in ischemia-reperfusion injury. This report suggested that polystyrene beads from 1.5- to 6-μm diameter (dia.) may have unfavorable effects after pretreatment. Here, we investigated whether injection of polystyrene beads (3- and 6-μm dia.) as an artificial contaminant of intravenous fluid after lipopolysaccharide (LPS) injection affected mortality and organ damage in mice.
Mice were divided into four groups and injected: polystyrene beads only, LPS only, polystyrene beads 30 min after LPS, or saline. A survival study, histology, blood examination, and urine examination were performed.
The survival rate after LPS and polystyrene bead (6-μm dia.) injection was significantly lower than that of the other three groups. In the kidney sections, injured glomeruli were significantly higher with LPS and polystyrene bead injection than that of the other three groups. LPS and polystyrene bead injection decreased the glomerular filtration rate and led to renal failure. Inflammatory reactions induced with LPS were not significantly different between with or without polystyrene beads. Polystyrene beads were found in urine after LPS and polystyrene bead injection.
Injection of polystyrene beads after LPS injection enhanced glomerular structural injury and caused renal function injury in a mouse sepsis model.
输注液体可能会受到不同类型颗粒的污染，这些颗粒可能对健康造成潜在危害。大于微脉管的颗粒可能通过机械阻塞和炎症引起栓塞；然而，据报道，直径小于毛细血管的颗粒相对安全。在此背景下，有一份报告表明，直径小于毛细血管的聚苯乙烯珠会降低缺血再灌注损伤中的组织灌注。该报告表明，预处理后，直径为 1.5-6μm（直径）的聚苯乙烯珠可能会产生不利影响。在这里，我们研究了在注射脂多糖 (LPS) 后注射聚苯乙烯珠（3 和 6μm 直径）作为静脉输液的人工污染物是否会影响小鼠的死亡率和器官损伤。
将小鼠分为四组并进行注射：仅聚苯乙烯珠、仅 LPS、LPS 后 30 分钟注射聚苯乙烯珠或生理盐水。进行生存研究、组织学检查、血液检查和尿液检查。
LPS 和聚苯乙烯珠（6μm 直径）注射后的存活率明显低于其他三组。在肾脏切片中，与其他三组相比，LPS 和聚苯乙烯珠注射后受损的肾小球明显更高。LPS 和聚苯乙烯珠注射降低了肾小球滤过率并导致肾功能衰竭。LPS 诱导的炎症反应在有无聚苯乙烯珠注射之间没有明显差异。LPS 和聚苯乙烯珠注射后在尿液中发现了聚苯乙烯珠。
在 LPS 注射后注射聚苯乙烯珠增强了肾小球结构损伤，并在小鼠脓毒症模型中导致肾功能损伤。
