Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411 Iran.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411 Iran ; Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, 1417614411 Iran.
J Environ Health Sci Eng. 2015 Apr 16;13:31. doi: 10.1186/s40201-015-0183-1. eCollection 2015.
In recent years, enzymatic-assisted removal of hazardous dyes has been considered as an alternative and eco-friendly method compared to those of physicochemical techniques. The present study was designed in order to obtain the optimal condition for laccase-mediated (purified from the ascomycete Paraconiothyrium variabile) decolorization of Acid Blue 92; a monoazo dye, using response surface methodology (RSM). So, a D-optimal design with three variables, including pH, enzyme activity, and dye concentration, was applied to optimize the decolorization process. In addition, the kinetic and energetic parameters of the above mentioned enzymatic removal of Acid Blue 92 was investigated.
Decolorization of Acid Blue 92 was maximally (94.1% ± 2.61) occurred at pH 8.0, laccase activity of 2.5 U/mL, and dye concentration of 75 mg/mL. The obtained results of kinetic and energetic studies introduced the laccase-catalyzed decolorization of Acid Blue 92 as an endothermic reaction (Ea, 39 kJ/mol; ΔS, 131 J/mol K; and ΔH, 40 kJ/mol) with K m and V max values of 0.48 mM and 227 mM/min mg, respectively. Furthermore, the results of microtoxicity study revealed that the toxicity of laccase-treated dye was significantly reduced compared to the untreated dye.
To sum up, the present investigation introduced the Paraconiothyrium variabile laccase as an efficient biocatalyst for decolorization of synthetic dye Acid Blue 92.
近年来，与物理化学技术相比，酶辅助去除有害染料被认为是一种替代的环保方法。本研究旨在通过响应面法（RSM）获得漆酶介导（从子囊菌 Paraconiothyrium variabile 中纯化）对酸性蓝 92 （单偶氮染料）脱色的最佳条件。因此，采用三变量 D-最优设计，包括 pH 值、酶活性和染料浓度，来优化脱色过程。此外，还研究了上述酶法去除酸性蓝 92 的动力学和能量参数。
酸性蓝 92 的最大脱色率为 94.1%±2.61%，在 pH 值 8.0、漆酶活性 2.5 U/mL 和染料浓度 75 mg/mL 时达到最大。动力学和能量研究的结果表明，漆酶催化酸性蓝 92 的脱色反应是一个吸热反应（Ea，39 kJ/mol；ΔS，131 J/mol K；和 ΔH，40 kJ/mol），K m 和 V max 值分别为 0.48 mM 和 227 mM/min mg。此外，微毒性研究的结果表明，与未处理的染料相比，漆酶处理后的染料毒性显著降低。
总之，本研究介绍了 Paraconiothyrium variabile 漆酶作为一种有效的生物催化剂，用于去除合成染料酸性蓝 92。
