Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 14174, Iran.
J Hazard Mater. 2012 Mar 30;209-210:199-203. doi: 10.1016/j.jhazmat.2012.01.012. Epub 2012 Jan 11.
The ability of Paraconiothyrium variabile, a laccase producing ascomycete recently isolated from soil, was studied to eliminate chlorophenol derivatives in submerged culture medium. Among the tested compounds, ρ-chlorophenol (ρ-CP) and pentachlorophenol (PCP) were found to have minimum and maximum toxic effects, respectively, on the growth of the microorganism and at the same time high and low bioelimination percentages. The fungal strain was able to remove 86% of ρ-CP (with initial concentration of 40 mg l(-1)) and 56% of 2,4-dichlorophenol (2,4-DCP; with same concentration as ρ-CP) after 9 days of incubation while no elimination was observed in the presence of 2,4,6-trichlorophenol (2,4,6-TCP) and PCP. Monitoring of laccase production level in the fermentation broth together with pollutant removal confirmed the key role of this copper-containing oxidase in chlorophenol derivatives elimination. The type of laccase inducer (guaiacol) and its final concentration (250 μM) and also initial pH of the fermentation broth (pH=5.5) in the elimination of ρ-CP increased the final removal yield from 86% to 94.3%.
从土壤中分离出的外生菌根真菌变侧耳（Paraconiothyrium variabile）具有漆酶活性，研究其在浸式培养体系中对氯酚衍生物的降解能力。在所测试的化合物中，ρ-氯酚（ρ-CP）和五氯酚（PCP）对微生物的生长分别具有最小和最大的毒性效应，同时具有最高和最低的生物去除率。该真菌菌株在 9 天的培养时间内能够去除初始浓度为 40mg/L 的 ρ-CP 的 86%和初始浓度与 ρ-CP 相同的 2,4-二氯酚（2,4-DCP）的 56%，而在 2,4,6-三氯酚（2,4,6-TCP）和 PCP 存在的情况下则没有观察到去除。发酵液中漆酶产量的监测以及污染物的去除证实了这种含铜氧化酶在氯酚衍生物消除中的关键作用。漆酶诱导剂（愈创木酚）的类型及其最终浓度（250μM）以及发酵液初始 pH 值（pH=5.5）对 ρ-CP 的去除也提高了最终去除率，从 86%提高到 94.3%。
