利用下一代测序技术和相关物种的交叉扩增对洪堡柳（杨柳科）中的23个多态性SSR标记进行表征。
Characterization of 23 polymorphic SSR markers in Salix humboldtiana (Salicaceae) using next-generation sequencing and cross-amplification from related species.
作者信息
Bozzi Jorge A, Liepelt Sascha, Ohneiser Sebastian, Gallo Leonardo A, Marchelli Paula, Leyer Ilona, Ziegenhagen Birgit, Mengel Christina
机构信息
Unidad de Genética Ecológica y Mejoramiento Forestal, Instituto Nacional de Tecnología Agropecuaria (INTA), E.E.A. Bariloche, Modesta Victoria 4450, Bariloche 8400, Argentina.
Faculty of Biology, Conservation Biology Group, University of Marburg, Karl-von-Frisch-Str. 8, 35043 Marburg, Germany.
出版信息
Appl Plant Sci. 2015 Apr 7;3(4). doi: 10.3732/apps.1400120. eCollection 2015 Apr.
PREMISE OF THE STUDY
We present a set of 23 polymorphic nuclear microsatellite loci, 18 of which are identified for the first time within the riparian species Salix humboldtiana (Salicaceae) using next-generation sequencing.
METHODS AND RESULTS
To characterize the 23 loci, up to 60 individuals were sampled and genotyped at each locus. The number of alleles ranged from two to eight, with an average of 4.43 alleles per locus. The effective number of alleles ranged from 1.15 to 3.09 per locus, and allelic richness ranged from 2.00 to 7.73 alleles per locus.
CONCLUSIONS
The new marker set will be used for future studies of genetic diversity and differentiation as well as for unraveling spatial genetic structures in S. humboldtiana populations in northern Patagonia, Argentina.
研究前提
我们展示了一组23个多态性核微卫星位点，其中18个是首次利用下一代测序技术在河岸物种洪堡柳（杨柳科）中鉴定出来的。
方法与结果
为了对这23个位点进行特征描述，每个位点抽取了多达60个个体并进行基因分型。等位基因数量从2个到8个不等，每个位点平均有4.43个等位基因。每个位点的有效等位基因数量在1.15至3.09之间，等位基因丰富度在每个位点2.00至7.73个等位基因之间。
结论
这个新的标记集将用于未来对阿根廷巴塔哥尼亚北部洪堡柳种群的遗传多样性和分化研究，以及揭示其空间遗传结构。
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