Lauron-Moreau Aurélien, Pitre Frédéric E, Brouillet Luc, Labrecque Michel
Institut de recherche en biologie végétale, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, Canada.
Plants (Basel). 2013 Mar 27;2(2):203-10. doi: 10.3390/plants2020203.
Biomass produced from dedicated plantations constitutes a source of renewable energy and is expected to play an important role in several countries in the coming decades. The cultivation of woody crops such as willows therefore raises several environmental issues. In North America, several native willows are potentially interesting for biomass producers. Willow trees are diverse but few species used for environmental applications have been the object of molecular genetic studies. Based on the sequenced poplar genome, 24 microsatellite markers were assayed on five native North American willow species: Salix amygdaloides, S. discolor, S. eriocephala, S. interior and S. nigra. Polymorphic microsatellite markers were used to characterize the allele data on the shrub Salix eriocephala, a North American species with economic potential. Eleven markers amplified and confirmed the potential of this species. Analysis of samples from six populations in eastern Canada showed that all markers were variable as well as polymorphic in at least one population. The number of alleles per locus ranged from 1 to 9 (mean 2.95) and showed that these microsatellite markers can be used to assess genetic diversity of North American willow species.
由专用种植园生产的生物质构成了一种可再生能源，预计在未来几十年将在多个国家发挥重要作用。因此，柳树等木本作物的种植引发了若干环境问题。在北美，几种本土柳树对生物质生产者来说可能很有吸引力。柳树种类繁多，但用于环境应用的物种中很少有成为分子遗传学研究对象的。基于已测序的杨树基因组，对五种北美本土柳树物种进行了24个微卫星标记的检测：杏仁柳、变色柳、毛头柳、内陆柳和黑柳。利用多态性微卫星标记对具有经济潜力的北美物种毛头柳的等位基因数据进行了表征。11个标记扩增并证实了该物种的潜力。对加拿大东部六个种群的样本分析表明，所有标记在至少一个种群中都是可变的且具有多态性。每个位点的等位基因数量从1到9不等（平均为2.95），表明这些微卫星标记可用于评估北美柳树物种的遗传多样性。
