Gonçalves Ariany Rosa, Barateli Luciana Oliveira, de Souza Ueric José Borges, Pereira Ana Maria Soares, Bertoni Bianca Waléria, Telles Mariana Pires de Campos
Programa de Pós-graduação em Genética e Melhoramento de Plantas, Escola de Agronomia, Universidade Federal de Goiás, Goiânia, Goiás Brazil.
Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás Brazil.
Physiol Mol Biol Plants. 2020 Oct;26(10):2095-2101. doi: 10.1007/s12298-020-00876-1. Epub 2020 Sep 16.
In this study, we report the development and characterization of 15 new microsatellite markers for (Leguminosae) in order to support future analyses of genetic diversity in populations of this species. In screening with 48 individuals from three different populations of , we tested the amplification of 20 microsatellite loci, of which five are not useful for population genetic studies due to the lack of polymorphisms or amplification failures. For the final set of 15 loci, the number of alleles ranged from 2 to 15, with a total of 116 alleles. The expected heterozygosity ranged from 0.1219 to 0.8965, with an average of 0.6694 per locus. The combined probability of genetic identity ( = 8.12 × 10) and paternity exclusion ( = 0.99999) estimations showed that the loci may be useful to discriminate between individuals of Initial cross-amplification tests were satisfactory in three species of the genus : , and . This new set of markers will be a useful tool for population genetic studies, contributing to the knowledge about the evolutionary history of and, additionally, correlated species.
在本研究中，我们报告了为[豆科植物名称]开发并鉴定了15个新的微卫星标记，以支持该物种种群未来的遗传多样性分析。在用来自[豆科植物名称]三个不同种群的48个个体进行筛选时，我们测试了20个微卫星位点的扩增情况，其中5个由于缺乏多态性或扩增失败而对种群遗传学研究无用。对于最终选定的15个位点，等位基因数量从2到15不等，共有116个等位基因。预期杂合度范围为0.1219至0.8965，每个位点平均为0.6694。遗传同一性联合概率（=8.12×10）和父权排除概率（=0.99999）估计表明，这些位点可能有助于区分[豆科植物名称]的个体。最初在[豆科植物属的三个物种名称]：[物种一名称]、[物种二名称]和[物种三名称]中的交叉扩增测试结果令人满意。这套新的标记将成为种群遗传学研究的有用工具，有助于了解[豆科植物名称]以及相关物种的进化历史。
