Siwiak Marlena, Zielenkiewicz Piotr
Department of Bioinformatics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, Warsaw, 02-106, Poland.
Laboratory of Plant Molecular Biology, Faculty of Biology, Warsaw University, Pawinskiego 5a, Warsaw, 02-106, Poland.
Biol Direct. 2015 Apr 25;10:18. doi: 10.1186/s13062-015-0048-7.
Co-regulation of gene expression has been known for many years, and studied widely both globally and for individual genes. Nevertheless, most analyses concerned transcriptional control, which in case of physically interacting proteins and protein complex subunits may be of secondary importance. This research is the first quantitative analysis that provides global-scale evidence for translation co-regulation among associated proteins.
By analyzing the results of our previous quantitative model of translation, we have demonstrated that protein production rates plus several other translational parameters, such as mRNA and protein abundance, or number of produced proteins from a gene, are well concerted between stable complex subunits and party hubs. This may be energetically favorable during synthesis of complex building blocks and ensure their accurate production in time. In contrast, for connections with regulatory particles and date hubs translational co-regulation is less visible, indicating that in these cases maintenance of accurate levels of interacting particles is not necessarily beneficial.
Similar results obtained for distantly related model organisms, Saccharomyces cerevisiae and Homo sapiens, suggest that the phenomenon of translational co-regulation applies to the variety of living organisms and concerns many complex constituents. This phenomenon was also observed among the set of functionally linked proteins from Escherichia coli operons. This leads to the conclusion that translational regulation of a protein should always be studied with respect to the expression of its primary interacting partners.
基因表达的共调控已为人所知多年，并且在整体以及单个基因层面都得到了广泛研究。然而，大多数分析关注的是转录控制，而对于物理上相互作用的蛋白质和蛋白质复合体亚基而言，转录控制可能是次要的。本研究是首次提供关联蛋白间翻译共调控的全球规模证据的定量分析。
通过分析我们之前的翻译定量模型结果，我们证明了蛋白质产生速率以及其他几个翻译参数，如mRNA和蛋白质丰度，或一个基因产生的蛋白质数量，在稳定复合体亚基和派对中心之间是高度协调的。这在合成复合体构建模块时可能在能量上是有利的，并确保它们及时准确产生。相比之下，对于与调控颗粒和日期中心的连接，翻译共调控不太明显，这表明在这些情况下维持相互作用颗粒的准确水平不一定有益。
在远缘相关的模式生物酿酒酵母和智人中获得的类似结果表明，翻译共调控现象适用于多种生物体，并涉及许多复杂成分。在大肠杆菌操纵子的功能相关蛋白组中也观察到了这种现象。这导致得出结论，蛋白质的翻译调控应始终结合其主要相互作用伙伴的表达进行研究。
