Rotondano Tereza Emmanuelle de Farias, Almeida Herta Karyanne Araújo, Krawczak Felipe da Silva, Santana Vanessa Lira, Vidal Ivana Fernandes, Labruna Marcelo Bahia, de Azevedo Sérgio Santos, Ade lmeida Alzira Maria Paiva, de Melo Marcia Almeida
Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brasil.
Unidade Acadêmica de Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande, Patos, PB, Brasil.
Rev Bras Parasitol Vet. 2015 Jan-Mar;24(1):52-8. doi: 10.1590/S1984-29612015011.
This study assessed the occurrence of Ehrlichia spp., Babesia spp. and Hepatozoon spp. infections in 100 tick-harboring dogs from a semiarid region of the State of Paraíba, Northeastern Brazil. Blood samples and ticks were collected from the animals, and a questionnaire was submitted to dog owners to obtain general data. Blood samples were used to perform hemogram, direct blood smear and immunological and molecular hemoparasite detection. The 1,151 ticks collected were identified as Rhipicephalus sanguineus; direct smears revealed E. canis-like morulae in the monocytes of 4% (4/100) of the non-vaccinated female dogs, and 34% and 25% of the dogs tested positive for Ehrlichia canis by indirect immunofluorescence assay (IFA) and polymerase chain reaction (PCR), respectively. Blood smear examination revealed Babesia-suggestive merozoites in the erythrocytes of 2% (2/100) of the animals. Babesia vogeli was detected by PCR in ten animals (10%) and was correlated with young age (p = 0.007) and thrombocytopenia (p = 0.01). None of the animals showed Hepatozoon spp. positivity. These results indicate that E. canis is the main tick-borne canine pathogen in the study area and provide the first report of B. vogeli infection in dogs from Paraiba State.
本研究评估了巴西东北部帕拉伊巴州半干旱地区100只携带蜱虫的犬类中埃立克体属、巴贝斯虫属和肝簇虫属感染的发生情况。从动物身上采集血样和蜱虫，并向犬主发放问卷以获取一般数据。血样用于进行血常规、直接血涂片以及免疫和分子血寄生虫检测。采集的1151只蜱虫被鉴定为血红扇头蜱；直接涂片显示，4%（4/100）未接种疫苗的雌性犬的单核细胞中有犬埃立克体样桑葚体，分别有34%和25%的犬通过间接免疫荧光试验（IFA）和聚合酶链反应（PCR）检测犬埃立克体呈阳性。血涂片检查显示，2%（2/100）的动物红细胞中有巴贝斯虫样裂殖子。通过PCR在10只动物（10%）中检测到伯氏巴贝斯虫，且其与年轻（p = 0.007）和血小板减少（p = 0.01）相关。没有动物显示肝簇虫属呈阳性。这些结果表明，犬埃立克体是研究区域主要的蜱传犬类病原体，并首次报告了帕拉伊巴州犬类感染伯氏巴贝斯虫。
