Yutin Natalya, Kapitonov Vladimir V, Koonin Eugene V
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, 20894, USA.
Biol Direct. 2015 Apr 25;10:19. doi: 10.1186/s13062-015-0054-9.
Search of metagenomics sequence databases for homologs of virophage capsid proteins resulted in the discovery of a new family of virophages in the sheep rumen metagenome. The genomes of the rumen virophages (RVP) encode a typical virophage major capsid protein, ATPase and protease combined with a Polinton-type, protein primed family B DNA polymerase. The RVP genomes appear to be linear molecules, with terminal inverted repeats. Thus, the RVP seem to represent virophage-Polinton hybrids that are likely capable of formation of infectious virions. Virion proteins of mimiviruses were detected in the same metagenomes as the RVP suggesting that the virophages of the new family parasitize on giant viruses that infect protist inhabitants of the rumen.
在宏基因组序列数据库中搜索噬病毒体衣壳蛋白的同源物，结果在绵羊瘤胃宏基因组中发现了一个新的噬病毒体家族。瘤胃噬病毒体（RVP）的基因组编码一种典型的噬病毒体主要衣壳蛋白、ATP酶和蛋白酶，并与一种Polinton型蛋白引发的B族DNA聚合酶相结合。RVP基因组似乎是线性分子，带有末端反向重复序列。因此，RVP似乎代表了噬病毒体-Polinton杂种，可能能够形成感染性病毒粒子。在与RVP相同的宏基因组中检测到了 Mimiviruses的病毒体蛋白，这表明新家族的噬病毒体寄生于感染瘤胃原生生物居民的巨型病毒上。
