Wharton R P, Struhl G
Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Cell. 1989 Dec 1;59(5):881-92. doi: 10.1016/0092-8674(89)90611-9.
Mutations in the BicaudalD (BicD) gene lead to a global reorganization of the Drosophila body pattern such that the head, thoracic, and anterior abdominal segments are replaced by posterior abdominal segments and terminalia. We first provide evidence that the primary cause of this phenotype is the inhibition of two anterior factors, bicoid and hunchback, by mislocalized activity of the posterior determinant nanos. We then describe the isolation of the BicD gene and show that it encodes a coiled-coil protein similar to the carboxy-terminal portion of the myosin heavy chain. Finally, we find that BicD protein is uniformly distributed throughout wild-type oocytes but is concentrated at the anterior pole of BicD mutant oocytes together with ectopic nanos activity. Taken together, these results suggest that BicD encodes a cytoskeleton-like protein involved in transporting or anchoring the nanos morphogen within the oocyte cytoplasm.
双尾D（BicD）基因的突变会导致果蝇身体模式的全面重组，使得头部、胸部和腹部前段被腹部后段和尾节所取代。我们首先提供证据表明，这种表型的主要原因是后决定因子纳米蛋白（nanos）的错误定位活性抑制了两种前部因子，即双胸蛋白（bicoid）和驼背蛋白（hunchback）。然后我们描述了BicD基因的分离，并表明它编码一种卷曲螺旋蛋白，类似于肌球蛋白重链的羧基末端部分。最后，我们发现BicD蛋白在野生型卵母细胞中均匀分布，但在BicD突变型卵母细胞的前极与异位的纳米蛋白活性一起聚集。综上所述，这些结果表明BicD编码一种类似细胞骨架的蛋白，参与在卵母细胞细胞质中运输或锚定纳米蛋白形态发生素。
