采用液相色谱-串联质谱法同时测定大鼠血浆中多柔比星和姜黄素及其在药代动力学研究中的应用。
Simultaneous determination of doxorubicin and curcumin in rat plasma by LC-MS/MS and its application to pharmacokinetic study.
作者信息
Ma Wenzhuan, Wang Jinling, Guo Qiang, Tu Pengfei
机构信息
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China.
Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China.
出版信息
J Pharm Biomed Anal. 2015;111:215-21. doi: 10.1016/j.jpba.2015.04.007. Epub 2015 Apr 9.
A specific, sensitive and rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of doxorubicin and curcumin in rat plasma after intravenous administration. The analytes of doxorubicin and curcumin were extracted with methanol precipitation using glibenclamide as internal standard (IS). The chromatographic separation was performed on a C18 column with acetonitrile and 0.1% formic acid water as mobile phase and with gradient elution at a flow rate of 0.2 mL/min. Calibration curves were linear over the ranges of 2-8000 ng/mL for doxorubicin and 5-2000 ng/mL for curcumin (r > 0.99). The lower limit of quantification (LLOQ) was 2 ng/mL for DOX and 5 ng/mL for Cur. Finally, this developed method was successfully applied in the pharmacokinetic study of doxorubicin and curcumin in rats and evaluated the effects of curcumin on the absorption of doxorubicin after intravenous administration.
已开发并验证了一种特异性强、灵敏度高且快速的液相色谱串联质谱（LC-MS/MS）方法，用于同时定量静脉给药后大鼠血浆中多柔比星和姜黄素的含量。以格列本脲为内标（IS），采用甲醇沉淀法提取多柔比星和姜黄素分析物。色谱分离在C18柱上进行，以乙腈和0.1%甲酸水为流动相，流速为0.2 mL/min进行梯度洗脱。多柔比星的校准曲线在2-8000 ng/mL范围内呈线性，姜黄素的校准曲线在5-2000 ng/mL范围内呈线性（r>0.99）。多柔比星的定量下限（LLOQ）为2 ng/mL，姜黄素的定量下限为5 ng/mL。最后，该方法成功应用于大鼠体内多柔比星和姜黄素的药代动力学研究，并评估了姜黄素对静脉给药后多柔比星吸收的影响。
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