Ginther O J, Wolf C A, Baldrighi J M, Greene J M
Eutheria Foundation, Cross Plains, Wisconsin, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Eutheria Foundation, Cross Plains, Wisconsin, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Theriogenology. 2015 Jul 15;84(2):193-9. doi: 10.1016/j.theriogenology.2015.03.010. Epub 2015 Mar 18.
Hourly circulating concentrations of a PGF2α metabolite (PGFM), progesterone (P4), and LH were obtained from a reported project, and concentrations of nitric oxide (NO) metabolites (NOMs; nitrates and nitrites) were determined in eight mares. Unlike the reported project, hormone concentrations were normalized to the peak of the first PGFM pulse of luteolysis (early luteolysis), second PGFM pulse (late luteolysis), and a pulse after luteolysis. The duration of luteolysis was 23.1 ± 1.0 hours, and the peak of the first and second PGFM pulses occurred 6.5 ± 0.9 and 14.8 ± 0.8 hours after the beginning of luteolysis. Concentration of P4 decreased progressively within and between the PGFM pulses Changes were not detected in LH concentration in association with the PGFM pulses. Concentration of NOMs was greater (P < 0.05) at the peak of the PGFM pulse during early luteolysis (88.8 ± 15.0 μg/mL) than during late luteolysis (58.8 ± 9.0 μg/mL). Concentration of NOMs began to decrease (P < 0.05) 4 hours before the peak of the PGFM pulse of early luteolysis. Concentration began to increase (P < 0.05) an hour after the peak of the PGFM pulse of late luteolysis. An NOM decrease and increase was not detected during the PGFM pulse after luteolysis. On a temporal basis, results indicated that NO either is not required for luteolysis in mares or has a role in or responds only during late luteolysis. A caveat is that the relative contribution of the CL versus other body tissues to circulating concentrations of NOMs in mares has not been determined.
从一个已报道的项目中获取了前列腺素F2α代谢物（PGFM）、孕酮（P4）和促黄体生成素（LH）的每小时循环浓度，并测定了8匹母马体内一氧化氮（NO）代谢物（NOMs；硝酸盐和亚硝酸盐）的浓度。与已报道的项目不同，激素浓度被归一化到黄体溶解第一个PGFM脉冲的峰值（早期黄体溶解）、第二个PGFM脉冲（晚期黄体溶解）以及黄体溶解后的一个脉冲。黄体溶解的持续时间为23.1±1.0小时，第一个和第二个PGFM脉冲的峰值分别出现在黄体溶解开始后的6.5±0.9小时和14.8±0.8小时。在PGFM脉冲内和脉冲之间，P4浓度逐渐降低。与PGFM脉冲相关的LH浓度未检测到变化。早期黄体溶解期间PGFM脉冲峰值时的NOMs浓度（88.8±15.0μg/mL）高于晚期黄体溶解期间（58.8±9.0μg/mL）（P<0.05）。在早期黄体溶解PGFM脉冲峰值前4小时，NOMs浓度开始下降（P<0.05）。在晚期黄体溶解PGFM脉冲峰值后1小时，浓度开始上升（P<0.05）。在黄体溶解后的PGFM脉冲期间未检测到NOMs的下降和上升。从时间上看，结果表明，NO要么在母马黄体溶解过程中不需要，要么仅在晚期黄体溶解过程中起作用或产生反应。需要注意的是，尚未确定黄体（CL）与母马体内其他身体组织对循环NOMs浓度的相对贡献。
