Greene J M, Ginther O J
Eutheria Foundation, Cross Plains, Wisconsin, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Eutheria Foundation, Cross Plains, Wisconsin, USA; Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Theriogenology. 2015 Jan 15;83(2):213-21. doi: 10.1016/j.theriogenology.2014.09.010. Epub 2014 Sep 21.
Temporal relationships among circulating concentrations of nitric oxide metabolites (NOM), progesterone (P4), and luteinizing hormone (LH) within the hours of a PGFM pulse were studied during luteolysis in heifers. The peak of a PGFM pulse was designated Hour 0. All of the following increases and decreases were significant. Within a spontaneous PGFM pulse (experiment 1; n = 7), concentrations of P4 and LH decreased between Hours -1 and 0 and increased between Hours 0 and 1; NOM increased between Hours -1 and 2. In experiment 2, PGFM pulses were simulated by intrauterine infusion of PGF2α (PGF group, n = 6), and another group was also treated with acyline to inhibit LH secretion (acyline-PGF group, n = 6). Averaged over the two groups, concentration of P4 decreased between Hours -2 and 0, increased (rebounded) between Hours 0 and 1, and decreased after Hour 2. In the PGF group, concentration of LH decreased between Hours -2 and -0.5 and increased between Hour 0 and Hour 1.5 to a maximum at Hour 1.5; NOM decreased between Hours -2 and -1.5 and increased between Hours 0 and 1.5. In the acyline-PGF group, the effect of hour was not significant for concentrations of LH and NOM. The absence of an increase in NOM concentration when LH was inhibited is a novel finding. The hypotheses were supported that concentrations of LH and NOM are temporally related, and LH has a role in the increase in NOM within the hours of a PGFM pulse.
在小母牛黄体溶解过程中，研究了前列腺素F代谢物（PGFM）脉冲数小时内一氧化氮代谢物（NOM）、孕酮（P4）和促黄体生成素（LH）循环浓度之间的时间关系。PGFM脉冲的峰值被指定为第0小时。以下所有的增加和减少都是显著的。在自发的PGFM脉冲中（实验1；n = 7），P4和LH的浓度在第-1小时和第0小时之间下降，在第0小时和第1小时之间上升；NOM在第-1小时和第2小时之间增加。在实验2中，通过子宫内注入PGF2α模拟PGFM脉冲（PGF组，n = 6），另一组也用阿西立林治疗以抑制LH分泌（阿西立林-PGF组，n = 6）。两组的平均值显示，P4浓度在第-2小时和第0小时之间下降，在第0小时和第1小时之间上升（反弹），并在第2小时后下降。在PGF组中，LH浓度在第-2小时和-0.5小时之间下降，在第0小时和第1.5小时之间上升，在第1.5小时达到最大值；NOM在第-2小时和-1.5小时之间下降，在第0小时和第1.5小时之间上升。在阿西立林-PGF组中，时间对LH和NOM浓度的影响不显著。当LH被抑制时NOM浓度没有增加是一个新发现。以下假设得到了支持：LH和NOM的浓度在时间上相关，并且LH在PGFM脉冲数小时内NOM的增加中起作用。
