基于三种人体细胞系的粉尘和颗粒物水溶部分的潜在细胞毒性及其与金属（类）的关系。
Potential cytotoxicity of water-soluble fraction of dust and particulate matters and relation to metal(loid)s based on three human cell lines.
机构信息
School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China; Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University, Guangzhou 510275, PR China.
Croucher Institute for Environmental Sciences, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China; School of Chemistry & Environment, South China Normal University, Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, Higher Education Mega Center, Guangzhou 510006, PR China.
出版信息
Chemosphere. 2015 Sep;135:61-6. doi: 10.1016/j.chemosphere.2015.04.004. Epub 2015 Apr 22.
Hepatocellular liver carcinoma (HepG2), human skin derived keratinocyte (KERTr,) and lung epithelial carcinoma (A549) were employed in MTT assay to evaluate the cytotoxicity of water-soluble fraction of road dust, air-conditioning (AC) filter dust and PM2.5 via ingestion, dermal contact and inhalation. Their effects on cell growth were dependent on exposure time and concentration. The LC20s of PM2.5 for A549 cell were approximately one order of magnitude lower than those of road dust and AC filter dust for KERTr cell and HepG2 cell. The LC20s of aqueous extracts were negatively correlated to the water-soluble metal(loid)s contained in dust coarse particles (KERTr: p=0.004; HepG2: p<0.001). However, no significant correlation between soluble metal(loid)s and LC20s of PM2.5 was observed for A549 cell (p>0.05). Other water-soluble components in dust and PM might cause the cell hazards synergistically or additively with metal(loid)s.
采用 MTT 法，选用人肝癌细胞（HepG2）、人皮肤角质形成细胞（KERTr）和人肺上皮癌细胞（A549），通过经口摄入、皮肤接触和吸入方式，评估道路尘、空调（AC）滤网尘和 PM2.5 水溶性成分的细胞毒性。其对细胞生长的影响取决于暴露时间和浓度。对于 A549 细胞，PM2.5 的 LC20 值约比 KERTr 细胞和 HepG2 细胞的道路尘和 AC 滤网尘 LC20 值低一个数量级。水提物的 LC20 值与尘中粗颗粒的水溶性金属（类）（KERTr：p=0.004；HepG2：p<0.001）呈负相关。然而，对于 A549 细胞，未观察到可溶性金属（类）与 PM2.5 的 LC20 值之间存在显著相关性（p>0.05）。灰尘和 PM 中的其他水溶性成分可能与金属（类）协同或累加引起细胞危害。
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